复方黄连处理后HNE1鼻咽癌细胞移植瘤的基因表达(英文)

Gene expression in tumor tissue transplanted with HNE1 nasopharyngeal carcinoma cell after treatment with Coptis chinensis

背景:复方黄连能够抑制鼻咽癌细胞移植瘤的生长,但其作用的机制并不很清楚。目的:探讨复方黄连抑制鼻咽癌细胞移植瘤生长的机制。设计:随机对照的实验研究。地点、材料和方法:实验在中南大学湘雅医学院动物实验中心完成。实验选用10只Balb/C裸鼠,鼠龄3～4周。饲养1周后,在每只裸鼠的前腋下接种0.5mL(0.5×107个)HNE1细胞。移植瘤形成后,根据移植瘤的大小,按数字法将裸鼠随机分成实验组和对照组,每组5只。实验组灌喂复方黄连,对照组裸鼠仅灌喂无菌水。从瘤组织提取RNA,经返转录荧光标记作为探针与cDNA芯片杂交后用激光共聚焦扫描仪分析基因表达并通过返转录-聚合酶链式反应技术进一步证实基因的表达。主要观察指标:复方黄连处理后HNE1鼻咽癌细胞移植瘤的基因表达。结果:复方黄连处理30d后,移植瘤明显减小,同时有335条基因的表达发生改变,即242条基因表达下调和93条基因表达上调。通过RT-PCR分析,6条基因证实为真正差异表达。其中,MAD3,H731和EGR-α基因mRNA表达增高,而TRAF5,P68激酶和CHK1基因mRNA的表达下降。结论:复方黄连能够抑制HNE1鼻咽癌裸鼠移植瘤的生长,对HNE1鼻咽癌裸鼠移植瘤的抑制作用可能与基因表达的改变有关。

BACKGROUND:The compound of Coptis chinensis can inhibit the growth of tumor transplanted with nasopharyngeal carcinoma(NPC) cell,but its mechanism remains not fully clear. OBJECTIVE:To investigate the mechanism of the inhibitory effect of compound Coptis chinensis on the growth of transplanted tumor with NPC cell. DESIGN:A randomized controlled trial study was conducted. SETTING,MATERIALS and METHODS:The experiment was completed in Animal Experiment Center of Xiangya Medical College,Central South University.Ten Balb/c nude mice of 3-4 weeks old were selected.After feeding for one week,0.5×107 /0.5 mL HNE1 cells were injected into the front limb of each nude mouse.Once the growth of tumor was visible,the nude mice were divided into experimental group and control group with 5 mice in each group according to the size of the transplanted tumor.The experimental group was treated with Coptis chinensis compound while the control group was only perfused with sterile water.RNA were isolated from the tumor tissues,hybridization with a cDNA microarray was performed with a probe consisting of a mixture of fluorescent labeled cDNAs reverse transcribed from RNA,and gene expression was analyzed by scanning the fluorescent intensity and further confirmed by RT PCR. MAIN OUTCOME MEASURES:Gene expression in tumor transplanted with HNE1 cell of NPC after treatment with Coptis chinensis compound. RESULTS:After treatment with Coptis chinensis for 30 days,the transplanted tumors were remarkably reduced in size.Meanwhile,expression of 335 genes was altered,of which 242 were down regulated and 93 were up regulated.Altered expression of 6 of these genes was confirmed by RT PCR.Among them,mRNA expressions of the MAD3,H731 and EGR α genes were increased,but those of TRAF5,p68 kinase and CHK1 were decreased. CONCLUSION:The compound of Coptis chinensis can inhibit the growth of tumor implanted with HNE1 cell of NPC in nude mice,which may be associated with the change in gene expression.