补肾健脾活血方药对实验性老年骨质疏松大鼠骨微结构的影响(英文)

Effect of formula medicines for strengthening kidney and spleen and promoting blood circulation on bone microstructure in rats with senile osteoporosis

背景:目前中医药防治骨质疏松的研究大多是针对绝经后骨质疏松的,其防治措施多以补肾为主,也有报道除补肾外,其他脏腑的调补不容忽视,但缺乏切实可信的对比研究。目的:探讨补肾健脾活血方药对老年骨质疏松的防治作用,寻求符合老年骨质疏松病机特点的防治方法。设计:完全随机对照实验研究。地点和对象:实验在湖北中医学院完成,对象为50只15月龄Wistar大鼠,雌雄各25只。干预:将大鼠随机分为5组:正常对照组、病理对照组、补肾活血组、健脾活血组、补肾健脾活血组。用地塞米松2.5mg/kg体质量肌注大鼠,2次/周、连续6周,复制老年骨质疏松模型。正常对照组和病理对照组灌服生理盐水,补肾活血组、健脾活血组、补肾健脾活血组分别灌服补肾活血方药、健脾活血方药、补肾健脾活血方药。6周后处死,取左股骨远端1/3制作脱钙病理切片,采用骨形态计量法方法观察。主要观察指标:相对骨体积、平均骨小梁宽度和单位面积骨小梁内骨细胞数。结果:正常对照组、补肾活血组、健脾活血组、补肾健脾活血组的相对骨体积(%)、平均骨小梁宽度(μm)均高于病理对照组(t=2.244～8.158,P<0.05～0.01);正常对照组、补肾活血组和补肾健脾活血组的单位面积骨小梁内骨细胞数(%)高于病理对照组(t=2.162～3.775,P<0.05～0.01);

BACKGROUND:At present,most studies on prevention and treatment of osteoporosis through traditional Chinese medicine focus on postmenopausal osteoporosis.In these studies,tonifying kidney is taken as the main method in preventing and treating osteoporosis.Besides suggesting tonifying kidney,some researchers have reported that nourishing and tonifying other zangfu organs should not be neglected in preventing and treating osteoporosis.However,no concrete and convincing control studies have been available. OBJECTIVE:To study the role of formula medicines for strengthening kidney and spleen and promoting blood circulation in the prevention and treatment of senile osteoporosis so as to explore effective methods of preventing and treating senile osteoporosis. DESIGN:A completely randomized controlled experiment. SETTING and PARTICIPANTS:The experiment was finished in Hubei College of Traditional Chinese Medicine.The subjects were 25 male Wister rats and 25 female Wister rats. INTERVENTIONS:The rats were randomly divided into 5 groups.Senile osteoporosis models were replicated by injecting dexamethasone intramuscularly at a dose of 2.5 mg/kg,twice a week,6 weeks consecutively.The normal control group and the pathological control group were given saline.KT BCP group,SI BCP group and KT SI BCP group were respectively administered orally the formula drugs for tonifying kidney and promoting the blood circulation,drugs for invigorating the spleen and promoting the blood flow,and drugs for tonifying kidney,invigorating the spleen,and promoting the blood circulation.They were all put to death 6 weeks after drug administration,and 1/3 of all rats' left distal thighs were collected to make decalcified pathological sections,and were observed by bone morphometry. MAIN OUTCOME MEASURES:The relative size of bones,the average width of bone trabecula,and the number of bone cells per unit of bone trabecula area were measured. RESULTS:The relative size of bones(%) and the average width of bone trabecula(μm) of normal control group,KT BCP group,SI BCP group,KT SI BCP group were all higher than those of the pathological control group(t=2.244-8.158,P< 0.05-0.01).The number of bone cells per unit of bone trabecula area of normal control group,KT BCP group,KT SI BCP group respectively was higher than that of the pathological control group(t=2.162-3.775,P< 0.05-0.01).The relative size of the bones and the average width of bone trabecula of KT SI BCP group were all higher than those of KT BCP group and SI BCP group(t=2.188-5.436,P< 0.05-0.01). CONCLUSION:Both the formular drugs for tonifying kidney and promoting the blood flow and the formula drugs for invigorating the spleen and promoting the blood circulation can effectively prevent and treat senile osteoporosis.The formula drugs that tonify kidney,invigorate the spleen,and promote the flow of blood showed an even better effect in preventing and treating senile osteoporosis than either KT BCP or SI BCP approach alone.