广州市交警谷胱苷肽转硫酶基因多态性研究

Study on GSTM1 genetic polymorphism in traffic police population in Guangzhou

目的 调查广州市交通警察人群谷胱苷肽硫酶GSTM1(GlutathioneS -transferasesM1)基因多态性分布。方法 采用人群普查和多重差异PCR(multi-differentialPolymerasechainreaction ,MD -PCR)方法对广州市交通警察外周血白细胞DNA的GSTM1基因缺失型进行检测 ,比较GSTM1基因在性别、籍贯、民族、家族肿瘤史、工作性质、工龄及每周接触汽车废气时间等方面的分布 ,并与其它地区人群进行比较。结果 广州市 2 0 93名在岗交通警察GSTM 1基因缺失率为 5 9 5 8% ,高于上海市区人群 4 8 86 % (P <0 0 5 ) ,低于云南籍肺癌人群 76 79% (P <0 0 5 )。性别、籍贯、民族、肿瘤家族史、工作性质、工龄每周接触汽车废气时间等方面没有显著性差异 (P >0 0 5 )。结论 研究人群GSTM1基因呈多态性分布 ,其等位基因和基因型率在研究人群内部分布无显著性差异。

Objective To investigate the polymorphism distribution of the GSTM1 genotypes in traffic police population in Guangzhou.Methods The epidemiological data were collected by a cross-sectional study and GSTM1 polymorhisms were determined by the multi-differential polymerase chiain reaction(MD-PCR) method using the samples of peripheral blood leukocyte DNA.Individuals were stratified according to their sex,native place,race,cancer family history,job and duration of exposure.The frequency of GSTM1-null genotype in traffic police population in Guangzhou was compared with that of other population in China.Results The frequency of GSTM1-null genotype came to 59.58% in the study population,which was higher than that of urban population in Shanghai normal population and lower than that of lung cancer population in Yunnan(P<0.05).However,no significant differences were found between GSTM1-mull genotype and sex,nationality,work group,family cancer history,length of service and work environment,exposed automobile more than 10 hours per week(P>0.05).Conclusion Distribution of the GSTM1 genes was polymorphic and GSTM-null genotype was similar in the characteristics of the study population.