低氧应激肽的核磁共振谱及快速检测方法研究

Investigate on nuclear magnetic resonance spectrum and fast detecting method for hypoxia stress peptide

目的 采用核磁共振仪器对低氧应激肽的指纹区信息进行研究 ,并建立快速检测方法为易感人群的筛选提供全新的检测手段。方法 对模拟高原环境 (减压低氧法 )的Wistar大鼠和急进高原患者分别采集血液行核磁共振氢谱 (NMR1H)检测 ,对所获表达特征进行归属鉴定 ;用自行配制的试剂对应激肽进行检测 ,对实验条件进行优化。结果 10 4只Wistar大鼠的血清在特定波长处有特征吸收峰 ;62只对照组仅有 2只有轻微表达 ,有显著性差异(P <0 0 1)。高原病组 43例 ,40例化学位移高表达 ,3例程度略低 (中度表达 ) ,健康对照组仅 2例有低表达 ,有显著性差异 (P <0 ,0 1) ;高原动物实验组与临床病例组血样在NMR1H谱图均有特征化学位移的表达。在快速检测方法中选择反应时间为 3 0min ,检测波长为 560nm。结论 在高原病易感者人群的体液中发现有低氧应激肽存在 ,其性质与目前国内外找寻的高原病相关物质特性均不相同 ,快速检测方法为部队兵员选择。

ObjectiveTo investigate dactylogram information of the h ypoxia stress peptide (HSP) with the Nuclear Magnetic Resonance (NMR) and to est ablish the fast method for detecting the HSP to provid the new measure for scre ening the crowd easy to sickness.MethodsTo detect the blood from the wistar rats treated in the hypoxic enviroment and the mountain sickness patients with the NMR,and de tect to the HSP with the reagent dispensed by ourselves,optimization the experim ent condition.ResultsCharacter absorption cusp was exited at the defin ite wave-length tn the serum from the 104 wistar rats,but only two was exited in the control group(62 cases),there was significant difference between the two gr oups.The cases of the chemistry migration expressed high of the mountain sicknes s and the control group were 40(43) and 2(43),showing significant difference bet ween the two groips(P<0.01).The NMRIH character chemistry migration expresse d both the group of the experiment of the mountain animal and the group of clini cal patients.The reaction time and wave-length were 30min and 560nm in the metho d of fast detecting.ConclusionThe HSP was exited in the body fluid of the mo untain sickness patients,the chapter of the HSP was different from the other sub stance as known;The method of fast detecting can provid the new medilum to selec t the soldier for the army and to screen the easy-sickned crowd.