直接免疫荧光技术对尖锐湿疣的诊断意义研究

A study on the diagnosis significance of direct immunofluorescence on Condyloma Acuminatum

目的 :了解直接免疫荧光技术对尖锐湿疣的诊断意义。方法 :2 0 0 3年 1月至 2 0 0 4年 5月在我院确诊的尖锐湿疣男性患者的疣体标本 10 0份作为病例组 ,同期在我院确诊为阴茎珍珠状丘疹的男性患者的病变部位标本 10 0份作为对照组 ,所有标本均接受直接免疫荧光和PCR检测。分析两种方法对CA诊断的灵敏度、特异度以及约登指数。结果 :DIF对CA诊断的灵敏度、特异度以及约登指数分别为 :6 8%、80 %、0 .4 8;PCR的为 97%、98%、0 .95。结论 :用DIF对CA作出诊断时需结合临床表现并慎重下结论。

Objectives: To know the diagnosis significance of direct immunofluorescence on Condyloma Acuminatum.Method: One hundred pieces of sample taken from male CA patients were selected as case group. Another hundred pieces of sample taken from male patients who were diagnosed as penis pearl papule were selected as control group. All patients treated in our hospital during January, 2003 to May, 2004. Every piece of sample was examined by DIF and PCR. Two methods were analyzed by counting and comparing sensitivity, specificity and Youden’s index. Result: Sensitivity, specificity and Youden’s index of DIF were 68%、80%、0.48 respectively.Those of PCR were 97%、98%、0.95 respectively. Conclusion: Clinical experiences must be taken into account when diagnosing CA by DIF.