鞘氨醇对人结肠癌细胞的抑制作用

Inhibitory effect of sphingosine on human colon HT-29 cells

目的 研究鞘氨醇对人结肠癌细胞的影响。方法 采用四甲基偶氮唑盐法 (MTT)、细胞核分裂指数和3 H -TdR掺入试验方法 ,所设剂量鞘氨醇分别为 0 ,0 82 ,1 6 3,3 12 5 ,6 2 5 ,12 5 μmol/L ,以二甲基亚砜 (DMSO)为溶剂对照。结果 鞘氨醇对HT - 2 9细胞的生长有明显抑制作用。在MTT试验中 ,不同浓度鞘氨醇对HT - 2 9细胞的7d抑制率分别为 18% ,4 5 % ,72 % ,87%和 89% ;在核分裂指数试验中 ,随着鞘氨醇剂量的增高 ,其核分裂指数明显降低 ;在3 H -TdR掺入试验中 ,可见随着鞘氨醇剂量的增高 ,3 H -TdR掺入到HT - 2 9细胞中明显的减少 ,与阴性对照组相比有显著性差异。结论 鞘氨醇对HT - 2 9细胞的生长增殖有明显抑制作用 ,这可能是鞘磷脂抑制结肠癌的机制之一。

Objective To study the influence of sphingosine on the growth of human colon HT-29 cells in vitro .Methods Using cell culture tests(MTT test,index of cellular mitosis,and \{\}\+3H-TdR incorporation test),to examine the growth of HT-29 cells at five concentrations(0,0.82,1.63,3.125,6.25,12.5 μmol/L)with a negative control (DMSO v/v).Results Sphingosine can inhibited the growth of HT-29 cells.After treatment with various sophingosine concentrations mentioned above for 7 days,the inhibition rates were 18%,45%,72%,87% and 89% respectively.With the increase of sphingosine concentrations,\{\}\+3H-TdR incorporation was decreased in HT-29 cells and the quantity of \{\}\+3H-TdR incorporation was significantly decreased as compared with the negative control ( P <0.05).Conclusion Sphingosine can significantly inhibit the growth of HT-29 cells,which was probably one of the preventive mechanisms of sphingomyelin for colon cancer.