摘要
目的 了解以东湖为源水的自来水中非挥发性有机污染物对细胞DNA的损伤作用。方法 应用单细胞凝胶电泳技术 ,对东湖源水不同水文期 (平水期、枯水期、丰水期 )的自来水中有机提取物所引起的Hela细胞DNA损伤作用进行观察。结果 (1)不同有机提取物剂量组 (5 0 ,10 0 ,2 0 0 ,4 0 0ml/2 5ml培养瓶 )引起Hela细胞DNA损伤与阴性对照组相比均有显著性差异 (P <0 0 5 ) ,并呈剂量 -反应关系 (P <0 0 5 )。 (2 )不同水文期水中非挥发性有机提取物对DNA损伤作用不同 ,总趋势是平水期 >枯水期 >丰水期。结论 以东湖为源水的自来水中非挥发性有机污染物对细胞DNA有断裂损伤作用 ,单细胞凝胶电泳技术的应用有助于环境水质的监测以及水中非挥发性有机提取物致癌及致突变机制的研究。
Objective To study the effects of the nonvolatile organic compounds in tap watr derived from Donghu Lake in Wuhan on DNA damage.Methods The nonvolatile organic compounds (NOCs) in water were abstracted with XAD-2 resin and the DNA damage of such NOCs in Hela cells were detected by using Signle Cell Gel Electrophoresis assay(SCGE).Results (1)Different orgainc extracts (50,100,200,400 ml/25 ml) from all samples of tap water could cause DNA strand breaks in Hela cells at certain range of concentrations,and there was positive dose-effect relationship( P <0.05);(2)A consistent potency for all the various hydrographic period,with decreasing activity in the order:average>low>high flow period.Conclusion The application of this assay would contribute to explain the mutagenic mechanism of the organic pollutants from tap water on the human body.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第7期784-785,共2页
Chinese Journal of Public Health
基金
国家"九五"重点科技攻关计划项目 (96 - 91 1 - 0 7- 0 2- 0 2 )
关键词
单细胞凝胶电泳
DNA损伤
自来水
非挥发性有机物
single cell gel electrophoresis
DNA damage
tap water
nonvolatile organic compounds