原发性高血压人群AGT基因多态性的研究

Epidemiological study on AGT polymorphism of essential hypertension

目的 运用分子流行病学的研究方法 ,测定在中国人群中原发性高血压病人血管紧张素原 (AGT)基因(A - 2 0C)多态性分布频率并探讨上述基因多态性及环境因素与原发性高血压之间的关系。方法 对安徽省西部原发性高血压病人进行流行病学调查 ,并运用多聚合酶链反应 (PCR)结合限制性内切酶方法 (RFLP)进行血管紧张素原基因 (A - 2 0C)多态性分布频率的测定 ,同时对影响高血压的诸多环境因素亦进行流行病学分析。结果 A等位基因的频率为 84 7% ,C等位基因的频率为 15 3% ,经卡方检验 (χ2 =0 5 8,P >0 0 5 )符合H -W平衡。AGT基因型与血压值的线性回归结果显示 ,在女性中AGT基因多态性 (A - 2 0C)的AC和CC基因型与AA基因型相比 ,SBP值显著升高 (P <0 0 5 )。结论 AGT(A - 2 0C)基因多态性测定尚属国内首次。与野生型 (AA)比较 ,AGT突变型 (AC +CC)对血压的影响以收缩压增高为主 ,而且以女性更为显著。

Objective To analyse the correlation between polymorphism of the angiotensiogen gene,environment factors,and essential blood hypertension.Methods To observe the blood pressure of the patients with essential hypertension patients in west of Anhui.Polymerase chain reaction(PCR) combined with restrictive fragment length polymorphism(RFLP) were used to detect genetype frequency and polymorphism for angiotensiogen gene(A-20C).Results The frequency of allele C and A accounted for 15.3% and 84.7% in the total populations.Through chi-square test,it meeted with H-W balance(χ 2=0.58, P >0.05).The linear regession of AGT genetype type with the baseline blood pressure value showed that SBP of the mutation type (AC+CC) group were higher than AA group( P <0.05),especially in female through linear Logistic regerssion( P <0.05).Conclusion It was the first time in China to report the AGT(A-20C) gene polymorphism and genetype frequency.High blood pressure was chiefly showed SBP increase for the total population with mutation type(AC+CC),especially among female.