膀胱癌中缺氧诱导因子和多药耐药相关蛋白表达的组织芯片研究

Tissue microarray study of HIF-1α and MRP gene in bladder cancer

目的 :利用组织芯片研究缺氧诱导因子 (HIF 1α)和多药耐药相关蛋白 (MRP)在膀胱癌中的表达及其相互间的关系。方法 :构建膀胱癌组织芯片 ,应用免疫组织化学SABC法检测 12 6例膀胱癌及正常组织中HIF 1α和MRP的表达。结果 :在 119例膀胱癌组织中 ,HIF 1α阳性表达率为 5 7.9% ,并随膀胱癌的临床分期升高而增加 (P <0 .0 1) ,尤其在G3 膀胱移行细胞癌中呈高表达 ,为 6 4 .6 % ,而且HIF 1α的表达与MRP的表达也密切相关 (P <0 .0 1)。结论 :HIF 1α的表达与MRP相关 ,抑制缺氧环境可提高膀胱灌注化疗效果。组织芯片技术在检测膀胱癌的原癌基因、抑癌基因及其相关蛋白质产物方面具有样本量大、检测指标多、耗时少和通量高等特点。

Purpose:Tissue microarray investigates the expression of hypoxia-inducible factor-1α(HIF-1α) and its relationship to multi-drug resistance protein (MRP) in bladder cancer. HIF-1α is a transcriptive factor that regulates genes involved in metabolism, angiogenesis, proliferation, and apoptosis of carcinoma.Method:Built up bladder cancer tissue microarray, Immunohistochemical streptavidin biotin peroxides complex(SABC) was used to examine the expression of HIF-1αand MRP in 126 cases of bladder cancer and Normal tissue.Result:In 119 cases of bladder cancer, positive rate of HIF-1α was 57.9 %, specially the positive rate of G 3 phase TCC was 64.6 %. HIF-1α expression increased as clinical stage increased (P< 0.01 ). The positive rate of MRP was 71.4 %. The relationship of HIF-1αand MRP was positive (P< 0.01 ).Conclusion:Expression of HIF-1α correlated with multi-drug resistance gene. Anti-HIF-1α may be helpful to chemotherapy of bladder cancer. Tissue chips technique has the advantage of high-throughput in the detection of bladder cancer related oncogenes, tumor-suppressor genes and their protein production, which can analyze larger amounts of specimens, more target genes, and spend less working time.