利用裸鼠建立人肝门部胆管癌肝门移植模型

Establishment of human hilar cholangiocarcinoma orthotopic tumor model in nude mice

目的 :应用肝门部胆管癌细胞系FRH及组织块法建立裸鼠人肝门部胆管癌肝门移植模型 ,观察肿瘤局部浸润和远处转移情况。方法 :2 0只裸鼠取上腹部切口 ,显露肝门部 ,取裸鼠皮下移植瘤边缘直径约 1mm组织块植入肝门部肝实质内。观察裸鼠生命体征的变化 ,定期处死裸鼠 ,取血查肝功 ,取肝门肿瘤、肝脏、胰腺、脾脏、胃肠、肺脏标本福尔马林液固定 ,苏木素 伊红染色后显微镜下观察 ,肝门移植瘤电镜观察。结果 :2 0只模型全部建立成功 ,成瘤率 10 0 % ,2周左右可触及肝门部有小结节 ,平均 4周出现黄疸 ,6周左右裸鼠出现明显恶液质。解剖后发现肝门部肿瘤侵及胆囊、肝门血管和腹腔淋巴结。胰腺、脾脏、胃肠、肺脏未见转移灶。结论 :肝门部胆管癌肝门移植模型成瘤率高 ,其转归接近临床 。

Objective:To establish a orthotopic model of human hilar cholangiocarcinoma in nude mice and investigate biological features of transplanting tumors.Methods:Upper abdominal incision was made.Liver hilar was exposed,the tumor mass(1mm×1mm×1mm)which was formed by injecting human hilar cholangiocarcinoma cell line FRH subcutaneously was orthotopically transplanted into liver hilar of 20 nude mice.Morphological and biological features,invasion and metastasis of the transplanted tumor were studied by light microscopy,electron microscopy and immunohistochemistry.Results:The successful rate of tumor transplantation was 100%.About 2 weeks later,there were small nodes at liver hillar,about 4 weeks later,jaundice was observed,and cachexia occurred at 6 weeks after transplantation in nud mice.After dissection,it was found that the tumor invaded to the gallbladder and metastasized to celiac lymphatic node and metastasized to liver hillar vessels.But no metastasis to pancreas,spleen,stomach,bowel and lungs was observed.Conclusion:Orthotopic transplantation model of human hilar cholangiocarcinoma is successfully established,its prognosis is similar to that of clinical tumor and may be very useful for the experimental research and clinical treatment.