血管内皮生长因子的表达和微血管密度与结直肠癌侵袭转移的关系

The relationship of the expression of vascular endothelial growth factor and microvessel density with invasion and metastasis of colorectal carcinoma

目的 :探讨血管内皮生长因子 (VEGF)的表达和微血管密度 (MVD)在结直肠癌侵袭和转移过程中的作用及其相互关系。方法 :采用免疫组织化学方法检测 6 1例结直肠癌组织中VEGF表达 ,用CD34染色标记血管内皮细胞 ,对癌组织中MVD进行计数。结果 :结直肠癌组织中VEGF阳性表达率为4 9 2 % ,MVD为 2 8 7± 12 9。在肿瘤坏死区周围或肿瘤浸润边缘VEGF表达较强 ,MVD较密集。VEGF表达及MVD与肿瘤浸润深度、淋巴结转移、肝转移及Dukes分期密切相关 (P <0 0 1) ,而与肿瘤大小、组织病理学类型无关 (P >0 0 5 )。高血管密度组 (MVD >2 9)淋巴结转移、肿瘤浸润浆膜和肝转移的发生率显著高于低血管密度组 (MVD≤ 2 9)。VEGF阳性表达组MVD显著高于阴性表达组 (P <0 0 1)。结论 :VEGF表达和MVD与结直肠癌侵袭和转移有关 。

Objective: To study the influence of vascular endothelial growth factor(VEGF) expression on angiogenesis,the relationship of VEGF expression and microvessel density (MVD) with invasion and metastasis of colorectal carcinoma.Methods: Sixty-one samples of colorectal carcinoma tissues were examined for VEGF expression by SABC immunohistochemical method and for the CD34 endothelial antigen by SP immunohistochemical method to highlight the new growth microvessels,the MVD of tumor was calculated.Results: The positive expression rate of VEGF was 49.2% and the mean of MVD was 28.7±12.9 (range from 6 to 55) in colorectal carcinoma tissues.The positive expression rate of VEGF and MVD were significantly correlated with the depth of invasion,lymph node metastasis,liver metastasis and Dukes’s stage(P<0.01).Incidence of tumor adventitia infiltration,lymph node metastasis and liver metastasis in group of higher MVD(>29) was significantly higher than that in group of lower MVD.MVD in group of VEGF positive expression was significantly higher than that in negative expression group (P<0.01).Conclusion:VEGF expression and MVD are significantly associated with invasion and metastasis and can be used as indicators of tumor behavior in colorectal carcinoma.