HLA-Ⅰ基因微卫星变异与宫颈癌相关性的分析

Genetic Instability of HLA-I Gene in Cervical Carcinoma

目的 对宫颈癌组织进行HLA Ⅰ基因微卫星变异的分析与作图。方法 采用 8个位于HLA Ⅰ区域的微卫星多态性标记分析 30例宫颈癌活检标本微卫星变异的情况。结果 微卫星变异总的发生率为86 7% (2 6 / 30 ) ,微卫星位点C32 11的杂合性缺失频率最高 ,可达 5 0 0 % (15 / 30 ) ,并发现了一个微小缺失区域 ;位点D6S2 5 8的MSI频率最高 ,达 4 0 0 % (12 / 30 )。结论 HLA Ⅰ基因微卫星变异在宫颈癌的发生和发展过程中起着重要作用 ,位点C12 5～C32 11之间的微小缺失区域可能存在有宫颈癌相关抑癌基因。

Objective To investigate the frequency of genetic instability at HLA-I loci in cervical carcinoma and the detailed deletion mapping in this region.Methods LOH and MSI of HLA-Ⅰ genes were analyzed in 30 paired blood and tumor samples using 8 microsatellite markers located in HLA-Ⅰ loci.Results Of the 30 cases, 26 (86.7%) showed genetic instability at one or more loci. Highest frequency of LOH was found at C3211 loci (50.0%). MSI was found in 20 out of 30 cases (66.7 %).Conclusion The data suggest that genetic instability of HLA-Ⅰ gene might participate in the carcinogenesis of cervical carcinoma. Meanwhile, the minimal deletion region might be defined between C125 and C3211, providing the evidence for cloning the tumor suppressor genes associated with cervical carcinoma.