2株海洋石油降解细菌的降解能力

Isolation and Identification of Two Marine Bacteria with Hydrocarbon- Biodegradation Activity

为实施海洋石油污染的生物治理 ,从厦门储油码头油污水中分离得到了 2株石油降解菌 .它们能够在以柴油、萘或芘为唯一碳源的培养基中生长 ,并适应于海洋环境的温度、pH和盐度 .它们对萘都有很强的降解能力 ,3d内降解率可达 87 5 3%和84 0 1 % ;7d内对芘降解率分别为 8 35 %、5 37% .经 1 6SrDNA同源性分析表明 2菌株之间及其与施氏假单胞菌的同源性皆为 99% ;Biolog生化鉴定也表明它们为不同的菌株 .通过兼并PCR扩增 ,序列分析发现 2种菌编码完全相同的萘双加氧酶基因 ,该基因与其它假单胞菌的萘双加氧酶大亚基有

Two bacterial strains, able to degrade both diesel fuel and PAHs, were isolated from the oil sewage of oil storage dock in Xiamen. Analysis of 16S rDNA sequences showed that both strains had high homology (99%) with Pseudomonas stutzeri and should be classified to this species. But Biolog analysis showed they were quite different strains. Tests of temperature,salinity,pH on cell growth suggested that they were from marine environment. Both showed high degradation activity to naphthalene,87 53% and 84 01% within 3 days;and slight activity to pyrene,8 35% and 5 37% within 7 days. And both harbored naphthalene dioxygenase genes of 98% identity with other those from Pseudomonas strains.