人血管生成素-1基因的克隆及表达载体的构建

Cloning of human angiopoietin-1 gene and construction of expression vector

【目的】克隆人血管生成素 1基因 ,并构建原核、真核表达载体。【方法】提取人骨髓中的总RNA ,反转录成cDNA ,利用巢式PCR技术扩出人血管生成素 1基因片段 ,并克隆到PQE 31、B7载体中。【结果】克隆了正确的人血管生成素 1基因 ,构建了PQE31Ang 1、B7Ang 1载体。【结论】人血管生成素 - 1基因的克隆及表达载体的构建 ,为进一步研究其功能、活性和应用奠定了基础。

To obtain the gene of human angiopoietin 1 and construct it's prokaryotic、eukaryotic expression vectors.Total RNA was isolated from human marrow,then the cDNA was amplified by reverse transcription.The fragment of human angiopoietinl gene was amplified by nested PCR and inserted into the PQE 31 and B7 vectors.The human angiopoietin 1 gene fragment was accurately cloned and the PQE31 Ang 1、B7Ang 1 vectors were constructed.[Conclusion]The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function、biological activity and application.