复发性念珠菌性阴道炎患者阴道分离白念珠菌对唑类药物的敏感性试验

Antifungal susceptibility testing of Candida albicans isolated from recurrent vulvovaginal candidiasis

目的:研究复发性念珠菌性阴道炎(RVVC)最主要的病原菌,即白念珠菌对唑类抗真菌药物的敏感性。方法:对从RVVC患者阴道中分离出的102株白念珠菌,采用美国国家临床实验标准委员会(NCCLS)抗真菌药物敏感试验分会公布的《酵母菌的液基稀释法抗真菌药物敏感试验参考方案》(M-27A方案)进行体外药敏试验。结果:氟康唑(FCZ)对102株白念珠菌的药敏试验示,37株白念珠菌最小抑菌浓度(MIC)≥64.000μg/mL,57株MIC>0.125μg/mL、<64.000μg/mL,8株MIC≤0.125 μg/mL。伊曲康唑(ICZ)对37株耐FCZ的白念珠菌药敏试验示,35株MIC≥16.000μg/mL,仅2株MIC<16.000μg/mL。结论:①NCCLSM-27A方案操作简便,MIC的一致性及重复性好,可作为体外抗真菌药敏感性试验的一种较好方法。②37株耐FCZ的白念珠菌对ICZ体外药敏试验结果表明,FCZ和ICZ可能具有相似的抗真菌作用机制。

Objective: To research the sensitivity and resistance of pathogenic yeasts, Candida albicans, isolated from recurrent vulvovaginal candidiasis (RVVC) to antifungal agents. Methods: The susceptibilities of the Candida albicans from RVVC to fluconazole (FCZ) and itraconazole (ICZ) were tested by NCCLS M-27A methods in vitro. Results: The susceptibility results of 102 strains of susceptibility testing of Candida albicans to FCZ showed there were 37 strains with MIC≤64.000 f44g /mL, 57 strains with MIC between 0.125 f44g /mL and 64.000 |j,g /mL, and 8 strains with MIC≤0.125 f44g /mL. The susceptibility results of 37 strains of the FCZ-resistant Candida albicans to ICZ showed there were 35 strains with MIC≤16.000 f44g/mL and only two strains with MIC<16.000 f44g/mL Conclusions: NCCLS M-27A micro-dilution method is reliable, reproducible and easy to use in the laboratory. Azole antifungal agents of FCZ and ICZ may have the similar mechanisms against Candida albicans.