眼镜蛇毒对大鼠脑桥某些核团一氧化氮合酶表达的影响

Effect of Naja naja atra Venom on Expression of Nitric Oxide Synthase in Certain Nuclei of Pons in Rat

目的 探讨眼镜蛇毒对大鼠脑桥某些核团一氧化氮合酶 ( NOS)阳性神经元表达的影响。 方法 采用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶 ( NADPH-d)方法 ,观察大鼠脑桥某些核团 NOS阳性神经元在眼镜蛇毒中毒组、生理盐水组、正常对照组之间的变化。 结果 蛇毒组大鼠脑桥的三叉神经运动核 ,面神经核 NOS阳性神经元比生理盐水组、正常对照组表达增强。 结论 眼镜蛇毒对脑桥的某些核团的 NOS阳性神经元表达有上调作用。

Objective To investigate the effect of Naja naja atra venom on the expressions of nitric oxide synthase(NOS) in certain Nuclei of Pons in Rat. Method The methods of nicotinamide adenosine dinucleotide phosphate diaphorase(NADPH-d) were used to observe the different expressions of NOS positive neurons in venom group,saline group and control group. Results Naja naja atra venom could increased the number of NOS positive neurons in motor nucleus of trigeminal and facial nerve nucleus,and the staining were more obviously than that of normal saline and control groups. Conclusions Naja naja atra venom coulde increase the expressions of NOS positive neurons in certain Nuclei of Pons in Rat.