Caveolin-1在载脂蛋白E基因敲除小鼠动脉粥样硬化形成中的变化

Changes of caveolin-1 in development of atherosclerosis in apo E-deficient mice

目的 :探讨caveolin 1在apoE基因敲除小鼠动脉粥样硬化形成过程中的变化。方法 :取正常和apoE基因敲除小鼠 (品系均为C5 7BL 6J)作为研究对象 ,分别观察不同周龄apoE基因敲除小鼠血清甘油三酯、总胆固醇、低密度脂蛋白、高密度脂蛋白的含量及主动脉横断面积、斑块面积的变化。免疫组织化学染色法对caveolin 1进行半定量及定位分析 ,Western blotting检测caveolin 1在主动脉的表达。结果 :apoE基因敲除小鼠的血清甘油三酯、总胆固醇、低密度脂蛋白水平与对照组比较显著升高 ,并随小鼠周龄增加而升高 ;斑块面积及斑块面积与主动脉面积的比值也随小鼠周龄增加而增大。免疫组织化学染色法显示 ,caveolin 1在实验组血管内膜表达呈阳性减弱 ,其程度随周龄增加有减少趋势。免疫印迹检测显示实验组caveolin 1的表达与对照组比较有所减弱 ,并与小鼠周龄呈负相关。结论 :apoE基因敲除小鼠主动脉内皮细胞caveolin 1表达下调 ,可能与其动脉粥样硬化形成有关。

AIM: To observe the changes of caveolin-1 in the development of atherosclerosis (AS) in apo E-deficient mice. METHODS: 40 male C57BL/6J mice and apo E-deficient mice were divided into two groups: control group and apo E-deficient mice group. The latter were further divided into 3 subgroups depend on the survival weeks (10, 20, and 30 weeks). The serum total cholesterol, triglyceride, high and low density cholesterol were determined. The areas of aortic wall and plaque lesion were calculated. Immunohistology and western blotting were used for analyzed caveolin-1. RESULTS: The serum levels of total cholesterol, triglyceride and low density cholesterol in the apo E-deficient mice group were more significantly increased than those in the control group, the plaque areas and the ratio of plaque areas and aortic areas also increased. But the expression of caveolin-1 in aortic wall of the apo E-deficient mice group was more significantly decreased than those in the control group. CONCLUSION: The expression of caveolin-1 in aortic wall of the apo E-deficient mice decreased, which may be related to the formation of atherosclerosis.