人参银杏合剂对抗大鼠脑急性低氧损伤的作用

Effects of ginseng and EGb mixture on acute hypoxia injury in rat brain

目的 :观察人参银杏合剂对模拟高原缺氧脑损伤的保护作用并探讨其机理。方法 :将成年SD大鼠随机分为常氧对照组、急性低氧组、人参银杏合剂加低氧组 ,分别观察各组大鼠海马CA1区锥体细胞层神经元形态的变化、脑组织含水量、Na+ K+ ATP酶活性、超氧化物歧化酶 (SOD)活性和丙二醛(MDA)含量。结果 :(1)缺氧 2 4h复氧 72h后 ,海马CA1区锥体层神经元形态发生明显变化 ,细胞数目减少 ,排列紊乱 ,突起消失 ,而人参银杏合剂加低氧组细胞损伤不明显 ,形态与对照组相似。 (2 )与对照组相比 ,急性低氧组的脑含水量显著增高 (P <0 .0 1) ,人参银杏合剂加低氧组脑含水量较急性低氧组明显降低 (P <0 .0 5 )。 (3)急性低氧组脑组织Na+ K+ ATP酶活性与对照组相比明显降低 (P <0 .0 5 ) ,人参银杏合剂加低氧组脑组织Na+ K+ ATP酶活性较低氧组明显升高 (P <0 .0 5 )。 (4)急性低氧组脑组织SOD活性与对照组比较有所下降 ,但无统计学意义 (P >0 .0 5 ) ,人参银杏合剂加低氧组SOD活性较急性低氧组明显上升 (P <0 .0 5 )。 (5 )急性低氧组脑组织MDA含量明显高于对照组 (P <0 .0 1) ,人参银杏合剂加低氧组脑MDA含量较急性对照组显著降低 (P <0 .0 1)。结论 :人参银杏合剂对高原缺氧脑损伤具有保护作用 ,其机理与其抗氧自?

AIM: To observe the protective effects and mechanisms of ginseng and EGb mixture on hypoxia brain damage under simulated plateau environment condition in rats. METHODS: Adult SD rats were randomly divided into 3 groups: normoxic control group (NC group), acute decompression hypoxia group (AH group), and ginseng and EGb mixture add decompression hypoxia group (MH group). Rats in the later two groups were exposed to simulated 8000 m high environment in the hypobaric chamber for 7 h. Water content, Na+-K+-ATPase activity, malondialdehyde (MDA), and SOD activity in cerebral tissue were measured. Ginseng and EGb mixture was administrated orally for 7 days in the MH group before decompression hypoxia, and the other two groups were administrated of the same volume water. RESULTS: Compared with the control group, cerebral water content was significantly increased in AH group, but it was more significantly decreased in MH group than that in AH group. Compared with the control group, Na+-K+-ATPase activity in cerebral tissue in AH group was decreased, but it was more significantly increased in MH group than that in the AH group. MDA level in AH group was higher than that in the control group, but it was more significantly decreased in MH group than that in AH group. SOD activity in AH group was decreased compared with the control group, but there was no significance at statistics. SOD activity in MH group was higher than that in AH group. CONCLUSION: Ginseng and EGb mixture has protective effects on hypoxic brain damage in simulated plateau environment condition. Its mechanisms are related to the antagonist effect on free radicals and membrane lipid peroxidation, the improvement of Na+-K+-ATPase activity, and energy metabolism of brain cells.