体外培养海马神经元生长过程中Nogo-A分布的变化

CHANGES IN Nogo-A DISTRIBUTION IN HIPPOCAMPAL NEURONS DURING GROWTH IN VITRO

目的 探讨Nogo A在神经元中的表达与神经元发育分化的关系 ,以推测Nogo A在中枢神经系统发育分化过程中的意义。 方法 采用怀孕 18d大鼠胚胎海马神经元体外高密度和低密度培养方法 ,应用免疫荧光细胞化学染色和Westernblot,观察Nogo A在体外培养的大鼠海马神经元的分布模式及其在不同生长阶段的变化。结果 Nogo A在海马神经元生长过程中均表达 ,主要分布在胞浆、胞膜和突起上。神经元突起形成过程中 ,Nogo A主要表达于突起的近端 ,在轴突上随着轴突的伸长逐渐表达于轴突远端和生长锥。Nogo A在成熟神经元网状的突起上呈串珠样分布。 结论 Nogo A在中枢神经系统中具有不同于抑制作用的其他功能 ,可能参与神经元突起生长、轴突投射等过程。

Objective To investigate the expression pattern and possible function of Nogo-A during neuronal growth. Methods E18 rat hippocampal neurons were primarily cultured both in high-density and low-density conditions. Immunohistochemistry and Western blot were performed to detect Nogo-A expression and distributional changes. Results Nogo-A was found in hippocampal neurons, mainly located in the cytoplasm, plasma membrane and neurites. It was detected at the proximal part of all neurites before axon formation. In axons, Nogo-A was enriched in the distal segment and axonal growth cone. In mature neurons, the fiber net work displayed a large number of Nogo-A immunoreactive varicosities. Conclusion The present results indicate that the neuronal Nogo-A may be involved in the process of neurite outgrowth and axonal projection.