大鼠杏仁内侧核NADPH-d阳性终末的起源

ORIGINS OF THE NADPH-d POSITIVE TERMINALSIN THE MEDIAL AMYGDALOID NUCLEUS OF THE RATS

目的 探讨一氧化氮合酶 (NOS)在杏仁内侧核传入投射神经元中的分布。 方法 霍乱毒素B亚单位 (CTb)逆行追踪和还原型尼克酰胺腺嘌呤二核苷酸脱氢酶 (NADPH d)组织化学双重染色相结合的方法。 结果双标神经元 (NADPH CTb)主要分布在中缝背核、蓝斑、臂旁核、导水管周围灰质腹外侧部以及杏仁复合体基底外侧核等神经核团。 结论 大鼠杏仁内侧核内的NADPH d阳性终末主要起源于上述核团 ,并且提示它们参与杏仁内侧核的功能调节。

Objective To estimate the effect of the NO on the medial amygdaloid nucleus(Me), we studied the origins of the NOS positive terminals in the Me. Methods Noergic afferent projection to Me was identified by a combined NADPH-d histochemical staning and retrograde CTb immunocytochemical method after microinjecting CTb into Me. Results The double labeled of neurons (NOS and CTb) were located in dorsal raphe nucleus, locus ceruleus, basolateral amygdaloid nucleus, parabrachial nucleus, ventrolateral part of periaqueductal gray.Conclution The NADPH-d positive terminals in the Me originates from the aforementioned nucleus, and may relate to the function of the Me.