表皮钙离子超微结构定位的研究(英文)

ULTRASTRUCTURAL LOCALIZATION OF EPIDERMAL CALCIUM GRADIENT BY ION CAPTURE CYTOCHEMISTRY

目的 观察钙离子在表皮的分布情况。 方法 裸鼠皮肤经丙酮处理后 ,采用离子捕获细胞化学法对裸鼠表皮钙离子的分布进行超微结构的定位。 结果 超微结构观察显示在正常的表皮中 ,基底层钙离子的浓度最低 ,由基底层到棘层钙离子的浓度逐渐增加 ,在颗粒层上部 ,钙离子的浓度最高。另外 ,裸鼠皮肤经丙酮处理后可导致表皮钙离子浓度梯度的丧失。 结论 表皮中存在一钙离子浓度梯度 ,离子细胞化学定位法是研究组织中钙离子分布的有效方法。

Objective To delineate the epidermal calcium ions distribution in normal and acetone-treated nude mouse skin. Methods acetone was applied on the nude mouse skin for 20*!min with cotton balls. The ion capture cytochemistry, i.e., the potassium oxalate-pyroantimonate method was employed to localize calcium ions in nude mouse epidermis for ultrastructural examination. Results Ultrastructural examinations demonstrate that abundant calcium ions displayed within the epidermis, with a low content of calcium in the basal and spinous layers, followed by a progressive increase with calcium content and reaching its maximal density within the outer stratum granulosum. Whereas, application of acetone to the nude mouse skin caused the loss of the epidermal calcium gradient.Conclusion The present results suggest that a calcium gradient exists within normal murine epidermis. Moreover, the ion capture cytochemistry is a potentially powerful investigative tool for the demonstration of epidermal ionic environment.