血府逐瘀汤在自发性高血压大鼠颈动脉血管胶原重构及逆转中的意义

Effect of Xuefuzhuyutang in Carotid Artery Vascular Remodeling in Spontaneous Hypertensive Rats

为探讨血府逐瘀汤在自发性高血压大鼠颈动脉血管间质胶原重构及逆转中的意义 ,选择 16周龄雄性自发性高血压大鼠 36只 ,分为自发性高血压大鼠组、伊贝沙坦组 [30mg (kg·d) ]和伊贝沙坦联合血府逐瘀汤 (联合用药 )组 3组 ;另选同龄雄性Wistar大鼠 12只作为对照 ;取颈总动脉切片行HE和苦味酸一天狼星红染色 ,分别检测血管壁厚 腔径比和胶原面积 ;应用放射免疫法测定血清中Ⅰ型和Ⅲ型前胶原的浓度。结果发现 ,自发性高血压大鼠组颈动脉壁厚 腔径百分比较对照组高 (14 .7%± 0 .5 %比 5 .9%± 0 .3% ,P <0 .0 5 ) ,伊贝沙坦组 (8.4 %± 0 .4 % )以及联合用药组 (7.6 %± 0 .5 % )较自发性高血压大鼠组下降 ;自发性高血压大鼠组胶原面积百分比较对照组高 (9.6 %± 0 .4 %比 4 .8%± 0 .3% ,P <0 .0 5 ) ,伊贝沙坦组 (8.2 %± 0 .4 % )较自发性高血压大鼠组下降 ,联合用药组 (6 .17%± 0 .38% )下降更明显 ;自发性高血压大鼠血清Ⅰ型前胶原、Ⅲ型前胶原和Ⅰ型前胶原与Ⅲ型前胶原比值均较对照组明显增高 (16 7.4± 18.7、6 4 .6± 7.6和 2 .6± 0 .3比 83.6± 10 .1、4 5 .3± 5 .6和 1.82± 0 .2 1,P <0 .0 5 ) ,伊贝沙坦组Ⅲ型前胶原下降明显 ,与对照组无统计学差异 ;

Aim To investigate the effect of Irbesartan and Xuefuzhuyutang in carotid artery vascular remodeling in the spontaneous hypertensive rats (SHR). Methods 12 SHR, 12 SHR treated with Irbesartan, 12 SHR with Irbesartan and Xuefuzhuyutang, 12 wistar rats were normotensive control group. The vascular volume fraction of Collagen and the ratio of wall and cavity were determined by picrosirius red and HE staining. The serum concentrations of procollagen type Ⅰ (PCⅠ) and procollagen type Ⅲ (PC Ⅲ) were measured by specific radioimmunoassays. Results Significant Fibrosis exists in carotid artery of SHR. After 20 week's Irbesartan treating, the vascular volume fraction of collagen and the ratio of wall/cavity were descended (8.19±0.40, 8.35±0.42 vs 9.64±0.41, 14.71±0.52, P<0.05), the effect with Irbesartan and Xuefuzhuyutang was better than the effect with Irbesartan only (6.17±0.38, 7.63±0.53 vs 8.19±0.40, 8.35±0.42, P<0.05). Baseline serum PCⅠ, PCⅢ and the ratio of PCⅠ/Ⅲ were increased in SHR (167.4±18.7, 64.6±7.6, 2.6±0.3 vs 83.6±10.1, 45.3±5.6, 1.82±0.21, P<0.05). The correlation between PCⅠ with volume fraction of collagen was positive (r=0.385, P<0.05), PCⅢ and the ratio of PCⅠ/Ⅲ with volume fraction of collagen were no significantly correlation in the SHR (r=0.089 and 0.107, P>0.05). Conclusion Xuefuzhuyutang was beneficial to reverse artery vascular collagen remodeling, and the serum concentration of PCⅠ can indicate the significance of the carotid artery vascular collagen remodeling in SHR.