抗极低密度脂蛋白受体多克隆抗体的制备及鉴定

Preparation and Characterization of Polyclonal Antibodies Against Very Low Density Lipoprotein Receptor

为制备抗极低密度脂蛋白受体的多克隆抗体 ,以极低密度脂蛋白受体配体结合域上特定片段的合成肽免疫新西兰家兔 ,收集免疫前后动物的血清 ,通过酶联免疫吸附法及免疫印迹法对血清中抗体进行分析和鉴定。结果发现 ,已获得高效价的抗极低密度脂蛋白受体血清 ,可利用免疫印迹法检测人、鼠组织和细胞中的天然极低密度脂蛋白受体及其亚型的蛋白。此结果提示 ,抗极低密度脂蛋白受体多克隆抗体的制备为从蛋白水平研究极低密度脂蛋白受体提供了有效的工具。

Aim To prepare the polyclonal antibodies against VLDL receptor and to characterize the antibodies. Methods Rabbits were immunized with polypeptide fragment of VLDL receptor as antigen. The blood serum of the immunized rabbits was collected. The quality of the antibodies were analyzed and characterized with ELISA and Western Blot. Results Rabbit against mouse and human VLDL receptor antibodies were obtained. The anti-serum have high title and can recognize the two subtypes of natural VLDL receptor through Western Blot. Conclusions The polyclonal antibodies against VLDL receptor were obtained, which provide a new tool to study the protein of VLDL receptor.