血管紧张素转化酶基因多态性对血清血管紧张素转化酶水平及血脂的影响

Effect of Angiotensin Converting Enzyme Gene Polymorphism on Level of Serum Angiotensin Converting Enzyme and Blood Lipid

为探讨血管紧张素转化酶基因多态性对本地人群高血压患者和正常人血清血管紧张素转化酶及血脂水平的影响,采用聚合酶链反应技术,对118例高血压患者和98例正常人的血管紧张素转化酶基因插入/缺失多态性进行分型,并检测血清血管祭张素转化酶活性及血脂含量。结果发现,高血压组血管紧张素转化酶三种基因型(缺失纯合子型、插入纯合子型和杂合子型)及插入/缺失等位基因的频率与正常对照组比较差异无统计学意义(X2=0.468,P=0.791;X2=0.379,P=0.538)。血清血管紧张素转化酶活性在三种基因型之间差异有显著性意义(F=17.107,P=0.000)。高血压组总胆固醇、低密度脂蛋白胆固醇、脂蛋白(a)高于正常对照组(P<0.05);高血压组三种基因型之间血脂各指标含量及正常对照组三种基因型之间总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇和载脂蛋白B含量差异有显著性意义(P<0.05)。此结果提示,血管紧张素转化酶基因多态性与血清血管肾张素转化酶活性及血脂含量有关,缺失纯合子型高血压患者血清血管紧张素转化酶活性最高且易患高脂血症。

Aim To explore frequency of angiotensin converting enzyme (ACE) gene insertion/deletion( I/D ) polymorphism and effect of ACE gene polymorphism on level of serum ACE and blood lipid in patient with essential hypertension and healthy subjects. Methods 216 subjects (98 heathy subjects and 118 hypertensives) were collected for the determination of the I/D genotype in intron 16 of ACE gene by polymerase chain reaction(PCR) and the level of serum ACE, total cholesterol (TC), triglycerides(TG), low density lipoprotein cholesterol (LDLC), high density lipoprotein cholesterol (HDLC), lipoprotein (a) [ Lp( a) ] , apolipoprotein AI (ApoAI), apolipoprotein B (ApoB). Results The frequency of I/D genotypes and D and I alleles of the ACE gene had no significant difference between hypertensives and healthy subjects (X2 = 0.468, P = 0.791; X2 = 0. 379, P = 0.538, respectively) . The level of serum ACE activity in deletion homozygotes (genotype DD) was the highest among three genotypes, and the lowest in insertion homozygotes (genotype II ), and intermediate in heterozygotes( genotype ID) (F= 17. 107, P = 0.000). Hypertensives had significantly higher TC, LDLC, Lp(a) than those of control group (P<0.0l). The level of blood lipid was significantly different among genotype DD, genotype ID, and genotype II in hypertensives group ( P < 0. 01). The level of TC, LDLC, HDLC, ApoB was significantly different among three genotypes in control group ( P < 0.05). Conclusions ACE gene polymorphism is associated with level of serum ACE and blood lipid. The level of serum ACE and blood lipid is higher in genotype DD than in genotype ID and genotype II .