白介素-1受体拮抗剂滴眼液治疗大鼠高危角膜移植免疫排斥反应

Experimental study on interleukin-1 receptor antagonist eye drops promoting high-risk corneal allografts survival

目的 观察白介素 1受体拮抗剂 (IL 1ra)滴眼液对高危角膜移植排斥反应的治疗作用。方法 SD大鼠为供体 ,Wistar大鼠为受体。缝线法诱导角膜新生血管后行角膜移植。对照组用生理盐水点眼 ,实验组分别用IL 1ra 1、3、5mg/mL点眼。同时设IL 1ra 1mg/mL结膜下注射组。观察植片的存活情况并检测植片中CD1阳性细胞。结果 与对照组相比 ,IL 1ra 3、5mg/mL组和结膜下注射组均可明显延长植片的存活时间 (P <0 0 5 )并减少植片中CD1阳性细胞的浸润。结论 IL

Objective To determine whether interlerkin-1 receptor antagonist (IL-1ra) eye drops can prolong corneal allografts survival in high-risk orthotropic transplantation in rat. Methods SD rats were used as donors and Wistar rats as recipients.Corneal neovascularization was induced by sutures.Three experimental groups received 1, 3 and 5 mg/mL IL-1ra eye drops respectively four times a day for 30 days.While 0.9% natural saline (NS) was used in the same way as negative control.1 mg/mL IL-1ra subconjunctival injection was also observed.All allografts were evaluated to determine rejection signs,and CD1 positive cells in grafts were examined using immuno-histochemistry. Results The mean survival time of corneal allografts were (5.80±0.79) days in the NS group, and (8.14±0.69) days in IL-1ra subconjunctival injection group.While the experimental groups showed a mean survival time of (5.89±1.05),(6.78±0.83),(9.00±2.36) days respectively. Compared with NS group,3 and 5 mg/mL IL-1ra eye drops and subconjunctival injection groups could significantly prolong the survival time of the grafts (P<0.05),but not in 1mg/mL group (P>05).CD1 positive cells in grafts were significantly reduced in different treated groups. Conclusion IL-1ra could prolong the survival time of corneal grafts by inhibiting the immune rejection of high-risk corneal transplantation and reducing the infiltration of CD1 positive cells.