HPLC法测定盐酸阿夫唑嗪片中盐酸阿夫唑嗪的含量

Determination of Alfuzosin hydrochloride in Alfuzosin hydrochloride Tablets by HPLC

目的 建立用HPLC法测定盐酸阿夫唑嗪片中盐酸阿夫唑嗪含量。方法 以盐酸特拉唑嗪为内标,采用Alltima-CN柱,流动相为 0 0 5mol·L-1磷酸二氢钠溶液(0 5mol·L-1的氢氧化钠溶液调pH至 5 4 ) -乙腈(78∶2 2);检测波长:2 4 4nm。结果与结论 盐酸阿夫唑嗪在 5～ 5 0 μg·ml-1范围内呈良好的线性关系,回收率为 99 8%,RSD =0 9% (n =9)。

Objective To establish a standard HPLC method for the determination of Alfuzosin hydrochloride in Alfuzosin hydrochloride Tablets.Methods Terazosin hydrochloride was used as internal standard,an Alltima-CN column was used with 0.05mol·L -1 NaH 2PO 4(pH 5.4,adjusted with 0.5mol·L -1 NaOH)-acetonitrile (78∶22) as mobile phase,and detected at a wavelength of 244nm.Results and Conlusion Alfuzosin hydrochloride was showed good linearity in the concentration of 5～50μg·ml -1,the average recovery was 99.8% with RSD of 0.9%(n=9).