大孔吸附树脂对杜仲叶中绿原酸、总黄酮的分离研究

STUDY ON ISOLATION OF CHLOROGENIC ACID AND FLAVONOIDS IN EUCOMMIA ULMOIDES LEAF BY MACROPOROUS RESIN

通过对静态吸附容量和洗脱效果的选择 ,从 10种大孔吸附树脂中确定出最适于杜仲叶中绿原酸、总黄酮分离的XDA- 5树脂。研究了用该树脂吸附分离杜仲叶中绿原酸和总黄酮的方法 ,得出以下结论 :1)杜仲叶绿原酸和总黄酮的最佳分离工艺为 :上柱液pH值为 2～ 3,静态吸附时间 8h ,绿原酸最佳洗脱剂为10 %～ 15 %乙醇溶液 ,黄酮洗脱剂为 5 0 %～ 70 %乙醇溶液 ,流速为每分钟流出液体积相当于吸附剂体积的8% ;2 ) 10 %～ 15 %乙醇洗脱液经真空浓缩后 ,其固形物得率为 4 .85 % ,绿原酸含量为 36 .6 5 % ;3) 5 0 %～ 70 %乙醇洗脱液经真空浓缩后 ,其固形物得率为 4 .98% ,总黄酮含量为 2 8.34%。

A specific macroporous resin,XDA-5 was selected from 10 different resins to adsorb and isolate chlorogenic acid and flavonoids from the leaves of Eucommia ulmoides through investigating the factors such as the capability of static adsorption and desorption.Thereafter,a methodological study was carried out on this resin.The following results were obtained.1)The favorable technological parameters for the isolation are:pH value 2-3 of the liquid extract to be isolated,static adsorption time 8 h,eluant for chlorogenic acid 10%-15% ethanol,for flavonoids 50%-70% (ethanol),elution rate 8% of the volume of the adsorbent per minute;2)The eluent rich in chlorogenic acid is concentrated by vacuum distillation at a yield of solid extracts 4.85%,content of chlorogenic acid in solid extract 36.65%,yield of chlorogenic acid 1.78%;3)The eluent rich in flavonoids is concentrated at a yield of solid extracts 4.98%,content of flavonoids 28.34%.