摘要
研究了自大枣提取香精后的残渣中获取多糖的制备工艺。以多糖得率及含量为指标 ,采用正交试验对大枣渣多糖的酶提工艺进行优选 ,以脱盐率和脱色率为指标 ,对大枣渣多糖精制纯化工艺进行考察。优选制备工艺为 :加胰蛋白酶 3% ,pH值 7,6 5℃温浸 1.5h ,再加木瓜蛋白酶 2 .0 % ,5 5℃温浸 1h。提取液经浓缩后通过AB- 8大孔吸附树脂柱脱色 ,再依次流经阴阳离子交换树脂脱盐 。
Method on preparation of polysaccharides from jujube(Ziziphus jujuba MILL.)fruit residue after extraction of jujube essence had been carried out. The enzyme method was optimized by orthogonal test with polysaccharides content and yield ratio as index.The purification process was examined with the rate of decolouration and desalination as index.The optimum conditions are: 3% of trypsin, 65 ℃, pH value 7 and extracted 1.5 h,then adding 2.0% of papain at 55 ℃ and extracted 1 h.The extracted solution was then decolorized through macro-reticular adsorption resin column followed by cation and anion exchange resin columns alternatively for desalting to obtain the purified polysaccharides.
出处
《林产化学与工业》
EI
CAS
CSCD
2004年第3期91-94,共4页
Chemistry and Industry of Forest Products
基金
河南省重大科技攻关资助项目 (984 0 2 12 0 0 )
关键词
大枣渣
多糖
Zizyphus jujuba MILL.residues
polysaccharide
