摘要
目的建立龙胆苦苷在Beagle犬血清中的含量测定方法,研究龙胆苦苷在Beagle犬体内的动力学规律。方法建立快速敏感的高效液相色谱检测方法,使用外标法,以龙胆苦苷静脉注射给药,Beagle犬含药血清样品经液-液萃取后在C18反相色谱柱上进行分离测定。结果龙胆苦苷血药浓度在0.1343～687.375μg·mL-1浓度范围内线性关系良好(r2=0.99993)。方法回收率为81.15%～97.99%,最低检测限为0.1343μg·mL-1。结论龙胆苦苷在Beagle犬体内的药动学过程表现为静脉内给药一房室模型,其中平均半衰期t1/2=1.1324h,消除速率常数Ke=0.5811h-1,表观分布容V=0.5316L·kg-1,消除率CL=0.3274L·kg-1·h-1。提示龙胆苦苷在体内经历了迅速分布、消除过程,在体内不易蓄积。
Objective To establish a method for measuring serum content of gentiopicroside in Beagle dogs and to explore its internal pharmacokinetic features. Methods The rapid and sensitive HPLC method was adopted as external standard. After intravenous injection of gentiopicroside, serum samples of the Beagle were collected and measured on C18 reverse-phase chromatographic column after liquid -liquid extraction. Results The linear range of gentiopicroside was 0.1343～687.375 μg·mL-1(r2=0.99993). The recovery was within 81.15 %～97.99 %. The limit of detection (LOD) was 0.1343 μg·mL-1. Conclusion The pharmacokinetic process of gentiopicroside can be described as one-compartment model with intravenous injection , t1/2 = 1.1324 h, Ke = 0.5811 h-1, V= 0.5316 L·kg-1, CL = 0.3274 L·kg-1·h-1. This indicates that gentiopicroside can be quickly distributed and eliminated and is not liable to be accumulated in the body.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第5期333-335,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
