谷氨酰胺预防幼兔全肠外营养相关肝损害的实验研究

Experimental study on the prevention of TPN-associated liver injury by glutamine

目的:探讨谷氨酰胺对幼兔全肠外营养相关肝脏损害的保护作用。方法:将43只幼兔分为正常对照组(n=12)、PN-10d组(n=18)和Gln-PN组(n=13)等3组,对各PN组动物连续24h输注营养液,10d后比较各组的肝功能、光镜和电镜下的病理变化。结果:Gln-PN组血胆汁酸和直接胆红素水平较PN-10天组显著下降;肝脏无胆汁淤积样改变。结论:谷氨酰胺可以减轻全肠外营养相关的肝脏损害。

Objective To study the preventive role against TPN associated liver injury by glutamine. Methods Forty-three New Zealand rabbits (6-8 days old) were divided into 3 groups: control group (n=12), PN-10 d group ( n=18) and Gln-PN group [using Ala-glutamine 400mg/(kg·d), n=13]. By the end of the experiment, blood biochemistry analysis and the histologic examination were performed. Results ①The serum level of direct bilirubin and bile acid in PN-10 d group were significantly higher than in the other groups. The light microscopy in the PN-10 d group demonstrated cholestatic changes or hepatic steatosis. Electron microscopy showed disappearance of microvilli, swollen mitochondria, and even occasional apoptotic changes in biliary ductules. ②The histologic changes were obviously in the Gln-PN group less evident than in the PN-10 d group. Conclusions Glutamine can help prevent and decrease TPN-associated liver injury in new-born rabbits.