梅花鹿生长激素基因的克隆及序列分析

Cloning and Sequencing Analysis of GH-Ⅰ Gene in Sika Deer

对梅花鹿的生长激素基因I部分片段进行了克隆测序 ,序列长度为 14 6 6bp ,现已将该序列提交到GeneBank上。GeneBank中的Blast分析表明 ,梅花鹿生长激素基因I与马鹿同源性为 99% ,与鼷鹿同源性为84 %。

In this paper,a partial fragment of growth hormone gene of spotted deer were cloned and sequenced. The length of the fragment is 1 465 bp.Now the sequence has been sent to GenBank with registration. Comparison the fragment sequence with that of Red deer and Tragulus javanicus growth hormone gene was carried out through blast of GenBank, the result showed that the homolgies of the nucleotide sequence was 99% with Red deer and 84% with Tragulus javanicus.