摘要
在实验室分离和纯化链霉亲和素的基础上对其性质进行了鉴定.在SDS-PAGE中链霉亲和素只有一条带,单体的分子量为14500Da,链霉亲和素的相对分子量约为58000Da;其等电点为5.9-6.2;结合生物素的活性为15.7U/mg.结果表明自制链霉亲和素为核心链霉亲和素,在纯度、活性等方面达到了理想的效果.
On the basis of previous isolation and purification of Streptavidin(SA) , the authors identify its characteristics. The molecular weight of SA was about 58000 Dalton by SDS-PAGE and its isoelectric point was 5.9-6.2 by IEF-PAGE. The averaged biotin - binding activity was 15.7U/mg. The results showed the SA purified by authors is core Streptavidin, which brings a-bout ideal effect as far as purity and activity is concerned and is thus reliable for immunological application and can be used as the substitute for imported SA.
出处
《上海师范大学学报（自然科学版）》
2004年第3期67-71,共5页
Journal of Shanghai Normal University(Natural Sciences)
基金
国家863计划项目(2002AA302210).
