摘要
目的研究苯并(a)芘对鲫鱼幼鱼肝脏总抗氧化能力(T-AOC)的影响。方法鲫鱼幼鱼分别暴露于3×10-4、3×10-3、3×10-2、3×10-1mg/LB(a)P,于染毒后1、3、6、12d用化学比色法测定T-AOC。结果染毒后第6天,3×10-1mg/L组的T-AOC水平明显低于其他各组(F=5.853,P<0.01),与3×10-2mg/L组比较,差异有显著性(t=7.114,P<0.01)。染毒后第12天,3×10-1mg/L组的T-AOC水平继续下降,而3×10-2mg/L组的T-AOC水平与染毒后第6天的检测结果比较也出现明显下降(t=7.544,P<0.01),与3×10-3mg/L组比较,差异有显著性(t=9.728,P<0.01)。在染毒后第12天,鲫鱼肝脏T-AOC水平与染毒剂量呈现明显的负相关关系(经spearman相关分析,r=-0.581,P<0.01)。3×10-1mg/L组的T-AOC水平随染毒时间的增加而出现明显的下降(经spearman相关分析,r=-0.830,P<0.01)。结论苯并(a)芘暴露可抑制鲫鱼肝脏的T-AOC水平,降低机体对环境毒物及致病因子的抵抗能力。
Objective To study the effects of B(a)P on total antioxidative capacity(T,AOC) in liver of Carassius auratus, and explore the toxic mechanism of B(a)P in aquatic ecotoxicology. Methods Test groups of younger Carassius auratus were exposed to 3×10-4, 3×10-3, 3×10-2 and 3×10-1 mg/L B(a)P respectively. After exposure for 1 d, 3 d, 6 d and 12 d, T,AOC in the fish liver was determined respectively by colorimetry. Results After exposure for 6 d, the level of T,AOC in 3×10-1 mg/L dose group was significantly lower than that in the other groups (F=5.853, P<0.01) and showed significant difference (t=7.114, P<0.01) compared with 3×10-2 mg/L dose group. After exposure for 12 d, the level of T,AOC in 3×10-1 mg/L dose group decreased continuously and the level of T,AOC in 3×10-2 mg/L dose group was significantly decreased compared to the result obtained after exposure for 6 d (t=7.544, P<0.01), the level of T,AOC in 3×10-2 mg/L dose group showed significant difference (t=9.728, P<0.01) with 3×10-3 mg/L dose group. Significant negative correlation (r=-0.581, P<0.01) between T,AOC and exposure dose was obtained after exposure for 12 d and the T,AOC level in 3×10-1 mg/L dose group decreased with the increasing of the exposure time(r=-0.830, P<0.01). Conclusion B(a)P could damage the antioxidative system and decrease the resistant capacity of organisms to environmental toxicants.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2004年第5期325-326,共2页
Journal of Environment and Health
基金
山东省科委基金资助项目(413774)
关键词
苯并(A)芘
总抗氧化能力
鲫鱼
Benzo(a)pyrene
Total antioxidative capacity
Carassius auratus