丰鲤及其双亲线粒体DNA限制性酶切图谱的研究

Study on Mitochondrial DNA of Feng Carp and Its Parents

采用密度梯度离心及RNase消化法制备并纯化了丰鲤(Fengcarp)、兴国红鲤(Xingguoredcarp)及散鳞镜鲤(Scatterscaledmirrorcarp)的肝脏线粒体DNA,用10种限制性内切酶HindIII、EcoRI、BamHI、XbaI、XhoI、PstI、BglII、SalI、BglI、PvuII进行了分析.丰鲤mtDNA相对分子质量约为9 88×106,大小约为16 49kb;兴国红鲤mtDNA相对分子质量约为9 89×106,大小约为16 50kb;散鳞镜鲤mtDNA相对分子质量约为9 87×106,大小为16 48kb.HindIII、EcoRI、BglI、BamHI、XbaI、XhoI、SalI、BglII、PstI及PvuII在丰鲤、兴国红鲤、散鳞镜鲤线粒体DNA分子上均分别有6、4、3、3、3、1、1、0、1和4个切点.根据单酶切及双酶切结果,构建了丰鲤、兴国红鲤、散鳞镜鲤mtDNA9种酶的限制性酶切图谱,结果表明丰鲤、兴国红鲤及散鳞镜鲤mtDNA间缺乏变异性.

The mtDNA from liver of Feng carp,Xingguo red carp and Scatter scaled mirror carp has been isolated and purified by density gardient centrifugation and RNase digestion.They were analyzed with 10 kinds of restriction endonuclease,which were Hind III,EcoR I,Bgl I,BamH I,Xba I,Xho I,Sal I,Bgl II,Pst I and Pvu II.The molecular weight of Feng carp mtDNA is 9.88×10~6,about 16.49?kb; and that of Xingguo red carp is 9.89×10~6,about 16.50?kb; and Scatter scaled mirror carp is 9.87×10~6,about 16.48?kb.The digested sites of the enzymes are 6,4,3,3,3,1,1,0,1 and 4,respectively.Based on results of single and double enzyme digestion,the restriction maps of Feng carp,Xingguo red carp and Scatter scaled mirror carp were established.The results showed that the genetic divergence among those 3 strains of common carp is exceptionally low.