摘要
研究了低温、高温、无碳源、秋水仙素和甘露醇 5种预处理对 5个辣椒品种小孢子离体培养前期存活率的影响 ,结果表明 ,4℃和无碳源处理对提高小孢子存活率作用明显 ,并且对供试品种都有作用。其中无碳源处理 2d对 83- 5 8的诱导效果最理想 ,存活率达 4 3.33% ,较对照提高 7倍多 ,较其它处理平均提高 2倍以上 ;另外75 - 7- 3- 1低温 (4℃ 2d)、秋水仙素 (1mg·L-12d)处理分别较对照提高 15 .5 %和 13.0 %。
The effects of low temperature,high temperature,sugar-free,colchicum and mannitol pretreatments on isolated microspore cultivation were studied. The result showed that 4 ℃ and sugar-free pretreatments significantly enhanced the survival rate of isolated microspore for all cultivars. The induction effect of sugar-free pretreatment on isolated microspore of 83-58 was the best. As compared with control, survival rate of isolated microspore on sugar-free medium increased 7.8 times and reached 43.33 % , and twice times higher than other pretreatments after treatment 2 days;pretreatments of 4 ℃ and 1 mg·L -1 of colchicum increased the survival rate of isolated microspore from 75-7-3-1 by 15.5 % and 13 % after treatment 2 days respectively as compared with control.
出处
《中国蔬菜》
北大核心
2004年第4期4-6,共3页
China Vegetables
基金
国家"863"计划 (2 0 0 1AA2 4112 1-9)
