摘要
以离体茎尖为试材 ,采用玻璃化法 ,对食用百合离体超低温保存技术进行了初步研究。结果表明 ,用2～ 3mm茎尖 ,在MS +0 .5mol·L-1蔗糖浓度的培养基上预培养 1～ 2d ,室温下植物玻璃化溶液 (PVS2 )处理 2 0min ,换入新鲜的PVS2 ,迅速投入液氮中 ,2d后取出 ,在 4 0℃水浴中解冻 2min ,再在 2 5℃水浴中解冻 10min ,用1.2mol·L-1蔗糖液体培养基洗涤 2 0min ,接种在 6 -BA 0 .5mg·L-1+NAA 0 .1mg·L-1+GA3 0 .3mg·L-1+蔗糖 30 g·L-1+琼脂 7g·L-1的MS培养基上 ,2 5℃弱光培养 1周后转为正常光下培养 ,2周后再生率达到 5 2 .6 %。
In order to study conservation technology of lily,the cryopreservation of lily shoot tips by vitrification was studied. The shoot-tips were preculture on MS medium with 0.5 mol·L -1 sucrose for 2 days and then the tip tissue with the size of 2-3 mm was dehyrated with a vitrification solution(100 % PVS 2) for 20 minutes at room temperature. After new vitrification solution was replaced.The materials were plunged into liquid nitrogen. After 48 h,the material was taken off from liquid nitrogen and thawed in 40 ℃ for 2 min and 25 ℃ for 10 min,and then was cultivated on MS+6-BA 0.5 mg· L -1 +NAA 0.1 mg·L -1 +GA 3 0.3 mg·L -1 +sucrose 30 g·L -1 +agar 7 g·L -1 .The material was cultured in low light intensity about 1 week and transfered to normal condition for about 2 weeks. The higest survival rate of tips after cryopreservation was 52.6 % .
出处
《中国蔬菜》
北大核心
2004年第4期11-13,共3页
China Vegetables
基金
2 0 0 2年度国家科技基础性工作专项"无性繁殖蔬菜种质资源的搜集和保存"
