固相萃取—气相色谱法测定动物组织中盐酸克伦特罗残留量

Determination of Clenbuterol in Meat Sample by Coupling Solid Phase Extraction with Gas Chromatography

建立了固相萃取—气相色谱分析动物组织中盐酸克伦特罗残留量的方法.采用固相萃取,分离富集动物组织中的盐酸克伦特罗.肉样的加标回收率在70%～80%;最低检出限为1μg/kg.盐酸克伦特罗的硅烷化衍生产物,采用气相色谱(ECD检测器)检测,其衍生物的峰面积与样品浓度在0.005～1.0μg/mL范围内呈良好的线性关系,线性回归系数大于0.999.

Solid phase extraction and gas chromatography have been developed for the determination of the residues of clenbuterol in meat sample. The recoveries of clenbuterol in meat sample are 70%～80%; the detection limit is 1 μg/kg for meat samples. The ECD detection method was used for the detection of the clenbuterol derivatives. There is a good linear correlation(the calibration coefficient is above 0.999) between the peak areas and concentration of clenbuterol in the range from 0.005 μg/mL to 1.0 μg/mL.