期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

农杆菌介导的胆碱脱氢酶基因(betA)转化小麦及影响转化效率的因素 被引量：8

Agrobacterium tumefaciens mediated choline dehydrogenase gene(betA) transfer to wheat and factors impacting on transformation
下载PDF
导出
摘要 用LBA4404/pCDH,Agl1/pUNN2和AglO(无质粒)3种菌株分别转化小麦品种济南177、99P、核生3号幼胚诱导的愈伤组织以及济南177的幼胚.其中以胚性愈伤组织为外植体,获得了转基因植株.PCR和PCR-Southern分析证实转化植株中包含了外源基因.通过染色体分析,发现胚性愈伤组织在农杆菌LBA4404侵染后形成的染色体削减比经Agl1侵染和未经感染的对照形成的染色体削减程度更大,甚至发现较多的染色体断片.分析了农杆菌菌株,材料的基因型,外植体类型,培养时间和温度以及筛选的时间和周期对于转化效率的影响. Immature embryos and embryogenic calli of wheat( Triticum aestivian L.) cultivars Jinanl77, 99P and Hesheng 3 were infected by three types of Agrobacterium LBA4404/pCDH, Agll/pUNN2 and AglO without plasmid. Transgenic plants regenerated only from embryogenic calli. PCR and PCR-Southern hybridization confirmed that transgenic plants contained foreign genes. Chromosomes of embryogenic calli infected were analysis. The elimination and breakage were more severe in the calli infected by LBA4404 than by AglO or control without infected.The factors impacted on transformation efficiency were also researched, including Agrobacterium types, wheat genotypes,explant types and period of selection etc.
作者 薛哲勇 贺晨霞 孙松 夏光敏
机构地区 山东大学生命科学学院
出处 《山东大学学报（理学版）》 CAS CSCD 北大核心 2004年第3期104-110,115,共8页 Journal of Shandong University(Natural Science)
基金 国家转基因专项资助项目(J99-037)
关键词 小麦 农杆菌转化 染色体消减和断裂 转基因植株 转化效率 Wheat transformation by Agrobacterium tumefacien chromosome elimination and fragmentation transgenic plants transformation efficiency
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献11

  • 1Deng W Y, Lin X Y, Shao Q Q. Agrobacterium Tumefaciens can Transform Triticum aestivum and Hordeum Vulgare of Gramineae[J].Science in China(series B),1990,33:27～33.
  • 2Cheng M,Fry J E,Pang S Z.Genetic transformation of wheat mediated by Agrobacterium tumefaciens[J].Plant Phsiol,1997,115:971～980.
  • 3Dillen W, Clercq D J, Kapila J, et al. The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer[J]. Plant Journal,1997, 12(6):1459～1463.
  • 4Uze M, Potrykus I, Sautter C. Factors Influencing T-DNA Transfer from Agrobacterium to Precultured Immature Wheat Embryos (Triticum aestivum L.) [J]. Cereal Research Communication,2000,28:17～23.
  • 5McCue K F, Hanson A D.Drought and salt tolerance towards understanding and application[J]. Tibteco, 1990,8:358～362.
  • 6郭光沁 夏光敏 李忠谊.小麦原生质体来源细胞的体细胞胚发生及植株再生[J].中国科学：B辑,1991,34(4):438-443.
  • 7Xia G M, Li Z Y, He C X , et al. Transgenic Plant Regeneration from Wheat (Triticum aestivum L.) Mediated by Agrobacterium tumefaciens[J]. Acta phytophysiologica Sinica, 1999, 25(1): 22～28.
  • 8Cheng, Z M, Schnurr J A, Kapaun J A, et al.Timentin as an alnative antibiotic for suppression of Agrobacterium tumefaciens transformation [J]. Plant cell report,1998,17(8):646～649.
  • 9Doyle J J,Doyle J L.Isolation of plant DNA from fresh tissue[J].Focus,1990,12:13～15.
  • 10薛哲勇,支大英,夏光敏,马秀玲,张慧,赵彦修.根癌农杆菌介导AtNHX1基因转化小麦[J].山东大学学报（理学版）,2003,38(1):106-109. 被引量：16

二级参考文献12

  • 1Hiei Y, Ohta S, Kumashiro T, et al. Efficient Transformation of Rice(Oryza sativa L.) Mediated by Agrobacterium and Sequence Analysis of the Boundaries of the T-DNA [J]. The Plant Journal, 1994,6(2):271-282.
  • 2Gould I, Devey M, Hasegawa, et al. Transformation of Zeamays L. Using Agrobacterium Tumefaciens and the Shoot Apex[J]. Plant Physiol,1991,95:426-434.
  • 3Deng W Y, Lin X Y, Shao Q Q. Agrobacterium Tumefadens can Transform Triticum aestivum and Hordeum Vulgate of Gramineae[J]. Science in China(series B), 1990,33:27-33.
  • 4Hess D, Dressier K, Nimmrichter R, et al. Transformation Experiments by Pipetting Agrobacterium Tumefaciens into the Spikelets of Wheat (Triticum Aestivum L.) [J]. Plant Sci,1990,72:233-244.
  • 5Cheng M,Joyce E F,Pang S Z.et a1.Genetic Transformation of Wheat Mediated by agrobacterium Tumefaciens[J].Plant Physiol,1997,115:971～980.
  • 6Xia G M,Li Z Y,He C X,et a1.Transgenic Plant Regeneration from Wheat(Triticum aestivum L.)Mediated by Agrobacterium Tumefaciers[J].Acta phytophysiologica Sinica 1999.25(1):22～28.
  • 7Peters N R,Ackennan S,Davis E A.A Modular Vector for Agrobacterium Mediated Transformation of Wheat[J].Plant molecular Biology Reporter 1999,17:323～331.
  • 8Neeta S.Chawla H S.Use of Silicon Carbide Filets for Agrobacteriumn-mediated Transformation in Wheat[J].Current Science,1999,76(11):1483～1485.
  • 9Murielle U,Ingo P,Christof S.Factors Influencing T-DNA Transfer from Agrobacterium to precultured Imrnature Wheat Embryos(Triticum astivum L.)[J].Cereal Research Communication,2000,28:17-23.
  • 10Junko U,Kei I,Teruo K,et al.Cloning and Sequencing of a Novel Na+,H+Antiporter Gene from Pseudoraonas Aeruginosa[J].Biochimica et Biophysica Actal998,1398:330～334.

共引文献15

同被引文献195

引证文献8

二级引证文献30

山东大学学报（理学版）
2004年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部