摘要
采用 3 个月胚龄的种胚播种形成的原球茎,初代培养时间在 2.5～3 个月之间换瓶转接,原球茎生长状况较好,分化形成的芽苗生长健壮;以 1/2MS 为基本培养基培养 30d,原球茎增殖率达 332%,MS 培养基更有利于分化,培养 2 个月分化发芽率达 90.6%;附加不同细胞分裂素原球茎的增殖率为 6-BA > KT > Ad+KT> Ad;NAA 浓度在 0～1.0mg/L,对原球茎增殖分化影响不明显。
Rapid multiplication of PLB and inducement into young plant is the key to realized commercial production of Phalaenopsis. The first generation of PLB grew well after 2.5 to 3 months cultivation, which were formed from 3 month-old embryo. The young plant grew vigorously after differentiation. The multiplication percentage of PLB was up to 332% after 30 days cultivation on 1/2 MS medium. MS medium was advantageous to differentiation. The descending of multiplication percentage of PLB was 6-BA, KT, Ad+KT, Ad, which supplemented with different cytokinins. NAA 0～1.0mg/L gave little effect on multiplication and differentiation of PLB.
出处
《亚热带植物科学》
2004年第3期42-44,共3页
Subtropical Plant Science
关键词
蝴蝶兰
原球茎
增殖分化
Phalaenopsis
protocorm-like body(PLB)
multiplication and differentiation
