人PTPRK基因在上皮性卵巢肿瘤中的表达及其意义

Expression and significance of human PTPRK gene in epithelial ovarian tumor

目的 探讨受体型蛋白酪氨酸磷酸酶(PTPRK)基因在良性、交界性和恶性上皮性卵巢肿瘤及正常卵巢上皮组织中的表达规律及其与卵巢癌的关系。方法 应用免疫组化间接法检测50例卵巢癌、13例良性上皮性卵巢肿瘤,14例交界性上皮性卵巢肿瘤及10例正常卵巢上皮组织中PTPRK基因的表达,采用Kaplan-Meier生存分析比较卵巢癌患者PTPRK蛋白表达与五年生存率的关系。结果 (1)良性、交界性上皮性卵巢肿瘤和卵巢癌组中,PTPRK基因的阳性表达率分别为53.9％、57.1％、18.0％。显著低于正常卵巢上皮组的表达率100％(P均<0.001)。卵巢癌组中PTPRK基因的阳性表达率显著低于良性上皮性卵巢肿瘤组(P<0.001)和交界性上皮性卵巢肿瘤组(P<0.01)。(2)卵巢癌高分化组中PTPRK基因的表达率为62.5％.显著高于中分化组(18.8％)和低分化组(6.3％)(P<0.01)。卵巢癌淋巴结无转移组PTPRK基因的表达率为27.3％。显著高于无阳性表达的淋巴结有转移组(P<0.05)。(3)PTPRK基因表达阳性组与表达阴性组的5年生存率分别为66.7％、46.7％,两组比较,差异无显著性(P>0.05)。结论 PTPRK基因表达缺失发生在上皮性卵巢肿瘤组织中,肿瘤分化程度越低或者淋巴结已有转移则该基因表达缺失越明显。PTPRK基因可能是一种肿瘤抑制基因,其不同程度的表达缺失与上皮性?

Objective To study the expression and significance of human protein tyrosine phosphatase receptor type kappa(PT-PRK) gene in benign, borderline and malignant epithelial ovarian tumor and normal ovarian epithelial tissue. Methods The expression of human PTPRK gene was investigated by immunohistochemical method in 50 patients with ovarian carcinoma, 13 patients with benign epithelial ovarian tumor, 14 patients with borderline epithelial tumor and 10 patients with normal ovarian tissue. Kaplan-Meier method was used to compare the expression of human PTPRK gene with five-year survival rate. Results The expression of human PTPRK gene was detected in significantly lower proportion in benign ovarian tumor(53. 9%),borderline tumor (57. 1%) and ovarian carcinoma (18%)than that in normal ovarian tissue(100%) (P<0. 001). Moreover, the expression of human PTPRK gene in ovarian carcinoma is lower than that in benign ovarian tumor (P<0. 001) and borderline tumor(P<0. 01). There was a higher expression rate (62. 5%) in well differentiated group than those in moderately differentiated group (18. 8%) and in poorly differentiated group (6. 3%) (P<0. 01). No lymph node metastasis group had a higher expression rate (27.3%) than lymph node metastasis group without any expression of PTPRK gene (P<0. 05). The five-year survival rate in positive PTPRK expression group or negative PTPRK expression group was 66. 7% or 46. 7% , respectively. Kaplan-Meier analysis showed that the expression of human PTPRK gene was not linked with prognosis(P>0. 05). Conclusion The lower expression of human PTPRK gene occurred in epithelial ovarian tumor, especially in ovarian carcinoma with poorly differentiated grade or with lymph node metastasis. Human PTPRK gene may be a tumor suppressor gene and correlative with the occurrence and development of epithelial ovarian tumor.