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RNA干扰和肿瘤基因治疗 被引量：2

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摘要 1 RNAi现象的发现 1990年,来自美国的Napoli[1]和荷兰的Krol[2]分别报道将外源性紫色色素合成基因查尔酮合酶(Chalcone Synthase,CHS)基因转入矮牵牛,试图产生出更深的紫色牵牛花,结果却发现花色素甙的合成发生了阻塞,大部分转基因植物开出了颜色斑驳或白色的花.检查白色花发现CHS基因mRNA的同步表达没有改变,而mRNA的转录比野生型植物减少50倍.导入基因和内源性基因均发生失活,这种现象被称为共抑制(co-suppression).随后多个植物学家亦在不同转基因植物中发现类似现象.与此同时,意大利Macino[3]领导的实验室将外源性类胡萝卜素基因albino-3导入红色面包霉菌(Neurospora crassa),发现约30%转化细胞的内源性的albino-3也受到了抑制,这种现象被他们称为静息作用(quelling).
作者 徐如祥 涂艳阳
机构地区 第一军医大学珠江医院神经外科全军神经医学研究所
出处 《肿瘤》 CAS CSCD 北大核心 2004年第5期512-513,共2页 Tumor
基金 国家自然科学基金资助项目(编号:30170961)
关键词 肿瘤/治疗 基因疗法 RNA 双链 RNA干扰
分类号 R73-362 [医药卫生—肿瘤]
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参考文献15

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同被引文献39

  • 1Eran Israeli,Eran Goldin,Oren Shibolet,Athalia Klein,Nilla Hemed,Dean Engelhardt,Elazar Rabbani,Yaron Ilan.Oral immune regulation using colitis extracted proteins for treatment of Crohn's disease: Results of a phase Ⅰ clinical trial[J].World Journal of Gastroenterology,2005,11(20):3105-3111. 被引量：8
  • 2陈长宝,青春,陆树良.siRNAs介导的基因沉默技术和相关应用的研究进展[J].上海第二医科大学学报,2005,25(11):1184-1187. 被引量：3
  • 3TERAZONO K,YAMAMOTO H,TAKASAWA S,et al.A novel gene activated in regenerating islets[J].J Biol Chem,1988,263(5):2111-2114.
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  • 5FUKUI H,FRANCESCHI F,PENLAND R L,et al.Effects of Helicobacter pylori infection on the link between regenerating gene expression and serum gastrin levels in Mongolian gerbils[J].Lab Invest,2003,83(12):1777-1786.
  • 6FUKUI H,FUJII S,TAKEDA J,et al.Expression of regⅠalpha protein in human gastric cancers[J].Digestion,2004,69(3):177-184.
  • 7SEKIKAWA A,FUKUI H,FUJII S,et al.Possible role of REGⅠalpha protein in ulcerative colitis and colitic cancer[J].Gut,2005,54(10):1437-1444.
  • 8GENG J,FAN J,WANG P,et al.REG1A predicts recurrence in stage Ta/T1bladder cancer[J].Eur J Surg Oncol,2009,35(8):852-857.
  • 9SCHMITTGEN T D,LIVAK K J.Analyzing realtime PCR data by the comparative C(T)method[J].Nature Protocols,2008,3(6):1101-1108.
  • 10LUHTALA N,PARKER R.LSM1over-expression in Saccharomy cescerevisiae depletes U6snRNA levels[J].Nucleic Acids Res,2009,37(16):5529-5536.

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肿瘤
2004年 第5期

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