泽兰有效部位对血小板聚集和血栓形成的影响(英文)

Effects of Active Fractions from Lycopus Lucidus L.F04 on Platelet Aggregation and Thrombus Formation

目的研究泽兰有效部位L .F0 4对血小板聚集和血栓形成的影响 ,以探讨其活血化瘀作用机理。方法用高分子右旋糖酐静脉推注造成大鼠血瘀证动物模型 ,观察泽兰L .F0 4对ADP诱导的大鼠体内血小板聚集以及体内动静脉旁路血栓、体外旋转环内血栓形成的影响。结果L .F0 4 0 .40 8g/kg、0 .2 0 4g/kg对模型组大鼠ADP诱导的体内血小板最大聚集率明显增加皆有显著的抑制 ,且呈剂量依赖关系 ;与对照组相比 ,血瘀模型大鼠体外血栓重量明显增加 ,长度仅有增加趋势 ,L .F0 4 0 .40 8g/kg、0 .2 0 4g/kg皆有抗血栓形成作用 ,L .F0 4 0 .40 8g/kg对血栓干重、湿重的减轻尤为明显 ;L .F0 4 0 .40 8g/kg、0 .2 0 4g/kg对实验性动静脉旁路血栓形成均有明显的抑制作用 ,抑制率分别为 2 7.41 %、2 7.1 4%。结论泽兰L .F0 4可显著抑制血小板聚集及体内。

Objective To study the effect of active fractions from Lycopus lucidus (L.F04) on platelet aggregation and thrombus formation so as to investigate its mechanism of promoting blood circulation and removing blood stasis. Method The effects of Lycopus lucidus L.F04 on platelet aggregation induced by ADP in vivo, and thrombosis of artery-vein by-pass and thrombus formed in circuitous loop in vitro were examined using the blood stasis animal model of rats made by injecting high molecular weight dextran (HMWD). Result The increase of maximum platelet aggregation rate induced by ADP in vivo in HMWD model was evidently inhibited with 0.408 g/kg or 0.204 g/kg of L.F04, and the effect showed a trend of concentration-dependence. As compared with the control, the thrombus weight in rat model of blood stasis increased clearly and its length showed only a trend of increase. Both 0.408 g/kg and 0.204 g/kg of L.F04 had the effect of resisting the thrombus formation, while 0.408 g/kg showed better effects of reducing the thrombus dry weight and wet weight. Both 0.408 g/kg and 0.204 g/kg of L.F04 could inhibit the thrombosis in artery-vein by-pass; the inhibition rates were 27.41 % and 27.14 % respectively. Conclusion Lycopus lucidus L.F04 could significantly inhibit platelet aggregation and thrombus formation.