摘要
以小麦新品种川农 17、12成熟胚为外植体进行离体培养 ,结果表明 :2mg/L 2 ,4 D有利于胚性愈伤组织形成 ,5mg/LKT有利于植株再生 (两品种植株再生率分别为 73 6 3% ,89 4 7% )。高浓度 2 ,4 D(4~ 8mg/L)虽不影响成愈率 ,但抑制植株再生。这种抑制作用能被KT拮抗。而KT的这种拮抗作用在 2mg/L 2 ,4 D条件下不能体现。
Mature wheat embryos of new wheat varieties Chuannong 17 and 12 were cultured in vitro. The results show that 2?mg/L 2,4-D is beneficial to the formation of totipotent calli. 5?mg/L KT is beneficial to plant regeneration (The regeneration frequencies of the two cultivars are 73.63%, 89.74% respectively). High concentration of 2,4-D (4~8?mg/L) has no bad effect on callus formation, but they inhibit plant regeneration. This kind of inhibition is resisted by KT, but in the initiation media supplemented with 2?mg/L 2,4-D this kind of resistance disappears.
出处
《四川农业大学学报》
CSCD
2004年第3期203-205,共3页
Journal of Sichuan Agricultural University
基金
四川省科技厅生物技术攻关项目
关键词
小麦
成熟胚
植株再生
植物激素
wheat
mature embryo
plant regeneration
phytohormone