2,4D、KT对小麦成熟胚愈伤组织形成、分化的影响

Effect of 2,4-D, KT on Callus Formation and Plantlet Regenerationfrom Mature Wheat (Triticum aestivum L.) Embryos

以小麦新品种川农 17、12成熟胚为外植体进行离体培养 ,结果表明 :2mg/L 2 ,4 D有利于胚性愈伤组织形成 ,5mg/LKT有利于植株再生 (两品种植株再生率分别为 73 6 3% ,89 4 7% )。高浓度 2 ,4 D(4～ 8mg/L)虽不影响成愈率 ,但抑制植株再生。这种抑制作用能被KT拮抗。而KT的这种拮抗作用在 2mg/L 2 ,4 D条件下不能体现。

Mature wheat embryos of new wheat varieties Chuannong 17 and 12 were cultured in vitro. The results show that 2?mg/L 2,4-D is beneficial to the formation of totipotent calli. 5?mg/L KT is beneficial to plant regeneration (The regeneration frequencies of the two cultivars are 73.63%, 89.74% respectively). High concentration of 2,4-D (4～8?mg/L) has no bad effect on callus formation, but they inhibit plant regeneration. This kind of inhibition is resisted by KT, but in the initiation media supplemented with 2?mg/L 2,4-D this kind of resistance disappears.