补肾宁心方对去卵巢兔动脉粥样硬化的保护作用

Protective Effect of Bushen Ningxin Recipe on Ovariectomized Rabbits with Atherosclerosis

目的研究补肾宁心方对去卵巢兔动脉粥样硬化形成的影响。方法 2 6只 3月龄新西兰雌兔随机分为正常组、假手术组、对照组 (卵巢切除 )、中药组 (卵巢切除并灌以补肾宁心方 ) ,除正常组外均于手术2周后给予高脂饮食 ,中药组同时灌胃给补肾宁心方中药 ,连续 3个月。各组于第 12周末测定血脂 (TC、TG、LDL C和HDL C)以及一氧化氮 (NO)水平 ;取主动脉行组织形态学观察以及在光镜和扫描电子显微镜下行病理学检查 ,利用图像分析仪计算动脉粥样硬化斑块内膜面积比和内膜中膜厚度比、面积比。结果 12周末除正常组外 ,各组动物血脂测定结果比较 ,差异无显著性。与对照组比较 ,中药组血清NO水平明显升高 ,主动脉硬化斑块面积与动脉内膜比值 ,内膜中膜厚度比、面积比均明显降低 ,主动脉病理变化减轻。结论补肾宁心方可抑制去势雌兔动脉粥样硬化形成 。

ObjectiveTo study the effects of Bushen Ningxin recipe (BNR) on atherosclerosis in ovariectomized rabbits. MethodsTwenty-six female New Zealand white rabbits of three months old were randomly divided into four groups, the normal group, the sham operation group, the control group (ovariectomized) and the treated group (ovariectomized and treated with BNR). All rabbits, except the normal group, were fed with high-cholesterol diet starting from two weeks after operation, and to the treated group BNR was given additionally for 3 months. Levels of blood lipid (TC, TG, LDL-C and HDL-C) and NO were measured at the end of the 12th week, and at the same time, histomorphological and pathological examination of aorta under light microscopy and scanning electron microscopy, and the ratio of atherosclerotic area of lesion/endothelium ratio (AL/AE) and thickness of endothelium/medium ratio (TE/TM) were estimated using image analyzer. ResultsNo significant difference was found in levels of blood lipid between groups, except those in the normal group. As compared with the control group, in the treated group, level of serum NO was higher, AL/AE and TE/TM were lower and the pathological changes of aorta was milder. ConclusionBNR could inhibit the atherosclerosis formation in ovariectomized rabbits, it showed protection on vascular endothelial cells.