连苯三酚法条件的优化及其在红薯梗活性评价中的应用

Study on Optimum O2 Scavenging Conditions by 1,2,3-trihydroxybenzene on Sweet Potato Stalk Activity

本实验中对连苯三酚法测定超氧阴离子自由基(O2)清除的条件进行了研究,确认了波长325nm,连苯三酚浓度1.875mmol/L,温度25℃为此体系最佳条件。应用于红薯梗实验结果表明,各有机溶剂梯度提取物对超氧阴离子自由基(O2)有一定的清除作用,其中以乙醚组分最强,约相当于同样浓度茶多酚活性的0.26。-·-·

In this experiment the optimum conditions for O2 free-radical scavenging assay by the method of the auto-oxidationof 1,2,3-trihydroxybenzene were investigated. They were confirmed as the wavelength, 325nm; concentration of 1, 2, 3-trihydroxybenzene, 1.875mmol/L and at 25℃. The results of sweet potato stalk activity assay showed that all extracts of sweetpotato stalks had acceptable O2 scavenging effects. Among all groups, ether-extracts showed the highest activity. However, itsactivity only corresponded to 0.26 time that of Tea Polyphenol at the same concentration(%W/W).