猪全层皮肤缺损创面新生上皮下肉芽组织的改建

Granule tissue remodeling under neoepithelium in pig's full thickness dermal wounds

目的 观察猪急性创面愈合过程中新生上皮下肉芽组织的改建过程及这种改建与以后可能形成的溃疡及瘢痕的关系。 方法 在 6只小型猪的背部各造成 8个直径为 4cm的圆形全层皮肤缺损创面。在创面形成后当日及 3,6 ,9,12 ,15 ,2 0 ,2 5 ,30 ,4 5d取创面及创面边缘的组织 ,进行HE染色、免疫组化染色和VanGieson苦味酸 -酸性品红染色 ,光镜下观察创面边缘新生上皮下和创面中心的总细胞数、成纤维细胞数、血管内皮细胞数和胶原纤维的含量及排列。 结果 创面愈合时间为 (2 9.3± 1.8)d ;伤后 9～ 2 5d ,创面边缘新生上皮下的总细胞数、成纤维细胞数和血管内皮细胞数较创面中心肉芽组织少 (P <0 .0 1) ;伤后 12～ 30d创面边缘新生上皮下的总细胞数和成纤维细胞数与创面愈合后 15d(伤后 4 5d)上皮下的细胞数比较 ,差异无显著性意义 (P>0 .0 1) ,创面边缘新生上皮下的胶原纤维的量和排列与创面愈合后 15d的上皮下相似。 结论在猪全层皮肤缺损创面愈合过程中 ,新生上皮下存在组织重建。

Objective To observe the granule tissue remodeling under neoepithelium in minitype pigs' full thickness dermal wounds and discuss the relation between the remodeling and possible ulcers or scars. Methods After the establishment of eight full-thickness dermal wound models with the diameter of 4 cm on the back of six minitype pigs, the specimens were collected from wound edge and wound center immediately, 3, 6, 9, 12, 15, 20, 25, 30 and 45 days, respectively after injury for HE staining, immunohistochemical staining and Van Gieson staining and then observed under light microscope to count the cell number, fibroblasts and vascular endothelial cells as well as evaluate the quantity and arrangement of collagens. Results The mean wound healing time was (29.3±1.8) days. After 9-25 days, the granule tissues in the wound center contained more cells, fibroblasts, collagen and vascular endothelial cells than those under neoepithelium of wound edge (P <0.01). There was no statistical difference between the total cell number and the number of fibroblasts under neoepithelium during wound healing (12-30 days) and those under neoepithelium 15 days after wound healing (P>0.01). Meanwhile, the collagen quantity and arrangement style of granule tissues under neoepithelium during wounds healing (12-30 days) assembled those under neoepithelium 15 days after wound healing. Conclusion Granule tissue remodeling exists during the healing of full thickness dermal wound.