纳米陶瓷支架的研制及其生物相容性评价

The experimental studies on manufacture and biocompatibility of nano-bioceramics scaffolds of tissue engineering bone

目的 制备纳米多孔羟基磷灰石 (HA)和磷酸三钙 (TCP)支架 ;研究新型陶瓷支架的生物相容性。方法 用Sel- gel法和直接沉淀法分别制备纳米级的HA及TCP粉体 ,采用有机泡沫浸渍法制备多孔陶瓷支架 ;采用人骨膜成骨细胞 (periosteal derivedosteoblastcells ,POB)与两种陶瓷支架的体外复合培养 ,观察细胞在材料上的生长及功能表达。结果 制备出具有良好孔隙结构和一定强度的纳米多孔陶瓷支架材料 ;人POB在HA和TCP表面良好的生长 ,并发挥成骨功能。结论 以HA、TCP的纳米级粉体为原料 ,采用有机泡沫浸渍法可制备出具有良好孔隙结构和较好力学强度的多孔陶瓷支架 ;新型多孔陶瓷支架有良好的生物相容性。

Objective To make porous nano-bioceramics and to study their biocompatibility as bone tissue engineering scaffolds. Methods The nanometer powders of hydroxyapatite(HA) and tricalcium phosphate(TCP) were prepared by Sol-gel methods and chemical deposition methods and the porous nano-bioceramics were made by foam sockage technics. The biocompatibility of new porous nano-bioceramics were evaluated by co-culture with human periosteal-derived osteoblast cells (POB) in vitro. The attachment and the growth of POB on the scaffolds were investigated by morphologic, proliferative abilities and osteogenic activity. Results HA and TCP porous nano-bioceramics scaffolds had a good network pore system and mechanic properties. POB can fully attached to, extended on both HA and TCP, and excreted extracellular matrix (ECM). Conclusion With the nanometer powders of HA and TCP, the porous nano-bioceramics can be produced by foam sockage technics. The new porous nano-bioceramics have good biocompatibility with POB.