人胎胰腺GnRH免疫反应细胞

GnRH immunoreactive cells in human fetal pancreas

目的 :探讨促性腺激素释的激素 (GnRH)免疫反应细胞在人胎胰腺的存在部位和数量变化。方法 :用免疫组织化学SABC法 ,对 3 7例第 1 0～ 3 2w人胎胰腺内的GnRH IR细胞进行观察 ,并用体视方法分析其数量变化。结果 :人胎胰腺GnRH IR细胞出现于第 1 3w ,其数密度随胎龄增加而增大 ;分布于胰岛及外分泌部的腺泡上皮、导管上皮细胞间。位于胰岛的GnRH IR细胞呈圆形、卵圆形或多边形。位于腺泡上皮细胞间的GnRH IR细胞多为锥体形 ,外分泌部的GnRH IR细胞均为开放型细胞。结论 :胰腺GnRH IR细胞于胚胎第 1 3w出现 ,广泛存在于内、外分泌部 ,其数量随胎龄增加而增加。

Objective: To investigate the distribution and the number of GnRH-immunoreactive (GnRH-IR) cells in human fetal pancreas. Methods: Using immunohistochemical technique and stereological method, the GnRH-IR cells in the pancreas of 37 human fetuses aging from 10-32th weeks were demonstrated and counted. Results: The GnRH-IR cells in pancreas were first seen in the 13th week of gestation, and they were localized in the pancreatic islets and the epithelium of pancreatic ducts and acinus. The shapes of GnRH-IR cells were round, ovoid or polygonal in the pancreaic islets and pyramidal in the exocrine acini respectively. The GnRH-IR cells in the ducts and acini were open type endocrine cells. Conclusion: The GnRH-IR cells in human fetal pancreas are widely distributed in both the endocrine pancreas and the exocrine pancreas. They appear first in the 13th week of gestation, and the number of pancreatic GnRH-IR cells tends to increase with fetal age.