可溶性肿瘤坏死因子受体与宫颈癌相关病原感染关系的研究

The relationship between STNFR and pathogens associated with cervical carcinoma

目的探讨肿瘤坏死因子(TNF-琢)、可溶性肿瘤坏死因子受体(STNFR)与宫颈癌发生、发展的关系及其STNFR与宫颈癌相关病原人乳头瘤病毒(HPV)、沙眼衣原体(CT)感染的关系。方法对62例宫颈癌用聚合酶链反应(PCR)法检测患者癌组织中HPV、CT,用双抗体夹心ELISA法检测患者血清中TNF-琢、STNFR-Ⅰ、STNFR-Ⅱ,并与正常的35例对照。结果宫颈癌患者癌组织中HPV、CT、HPV+CT感染率明显高于正常对照组(P<0.001);宫颈癌患者血清TNF-a、STNFR-Ⅰ、STNFR-Ⅱ以及CT感染阳性组STNFR-Ⅱ水平均高于阴性组(P<0.05)。结论TNF-琢、STNFR与宫颈癌的发生发展有关,STNFR水平的增高可能与宫颈癌HPV、CT感染有关,其发生原理有待进一步研究。

Objective To explore the correlation among tumor necrosis factor-?α (TNF-?α), soluble tumor necrosis factor receptor (STNFR) and the occurrence, progress of cervical carcinoma; to study the correlation of STNFR with infecting pathogens, such as human papillomavirus (HPV), Chlamydia trachomatis (CT) and their association with cervical carcinoma. Method HPV, CT were detected by PCR from the patient′s carcinomatous tissue;the serum TNF-?α, STNFR-Ⅰ, STNFR-Ⅱ were measured by sandwich ELISA in 62 cases of patients with cervical carcinoma, and 35 cases of normal women as the control. Result The positive rate of HPV, CT in the cervical tissue among the patients with cervical carcinoma was significantly higher than that among the control group (P<0.01). The serum TNF-?α, STNFR-Ⅰ, STNFR-Ⅱof cervical carcinoma patients were significantly higher than those of the control group (P<0.001), The serum STNFR-Ⅰ, STNFR-Ⅱ of the patients who infected by HPV, and STNFR-Ⅰ of the patients who infected by CT, respectively were higher than those who were not infected with HPV or CT in the patients with cervical carcinoma (P<0.01). Conclusion TNF-?α, STNFR are associated with the oncogenesis and development of cervical carcinoma.Increased serum STNFR levels seem to be related with the infection of HPV and CT in the patients with cervical carcinoma, its occurrence mechanism needs further investigation.