hu-RBC-SCID小鼠模型的建立及野鼠Babesia microti样原虫感染人红细胞的研究

Isolation of Babesia microti-like Parasites from Rodents in hu-RBC-SCID Mice

目的 用人红细胞 (RBC)替换SCID小鼠自身血循环红细胞而建立的人化小鼠模型 ,即hu RBC SCID模式小鼠 ,分离并测试野鼠Babesiamicroti(B .microti)样原虫对人红细胞的感染 ,以确定人巴贝斯原虫的感染来源。方法 用人O型红细胞注入先期去除自身血循环红细胞的NOD shi scid小鼠血循环中 ,辅以肌内注射兔抗小鼠血红蛋白生成素血清和大鼠抗小鼠RBC单克隆抗体 ,建成hu RBC SCID模式小鼠 ,接种野鼠感染B .microti的RBC。结果 hu RBC SCID模式小鼠的人RBC中 ,在接种感染B .microti野鼠的RBC后 ,出现巴贝斯样原虫 ,并大量增殖。RDNA序列比较发现两类为B .microti样原虫 ,一类与人的一致。结论 感染野鼠的两类B .microti样原虫均对人RBC具有高度感染性 ;hu RBC SCID模式小鼠的感染 。

Objective To isolate and identify piroplasms from rodents in Japan by using NOD/shi scid mice, and to test its nature to infect human RBC. Methods The rodents' blood specimens were inoculated into NOD/shi scid mice, whose circulating erythrocytes(RBC) had been replaced with human RBCs(hu RBC SCID mice). Results Babesia microti isolated from rodents proliferated within the human RBCs in the hu RBC SCID mice, and one type was identical to the parasites from human. Conclusion The isolates from rodents were demonstrated to be infective for human erythrocytes by inoculation into hu RBC SCID mice.